Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing, China.
Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing, China.
Int J Biol Macromol. 2020 Apr 1;148:1053-1060. doi: 10.1016/j.ijbiomac.2020.01.220. Epub 2020 Jan 23.
Vulvovaginal candidiasis (VVC) is one of the most common infections in women. The purpose of this study was to evaluate the ability of a water-soluble β-glucan salecan to protect against Candida albicans (C. albicans) vaginal infection. The model was reproduced with intravaginal inoculation of yeast blastoconidia in pseudoestrus mice. We found that mice that received salecan (0.5 mg per mouse) after infection had 85% fewer CFU than infected mice given saline. Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1. The analysis for vaginal microbial community composition at different taxa levels revealed that the bacterial flora composition in the vagina of the salecan-treated mice was similar to that of the uninfected mice, and distinguished from the infected mice. The vaginal lavages from the salecan treated group had more Enterococcus and its metabolite lactate. Our results suggest salecan might be a potential therapeutic agent for vaginal infection of C.albicans.
外阴阴道假丝酵母菌病（VVC）是女性最常见的感染之一。本研究旨在评估水溶性 β-葡聚糖 salecan 预防白色念珠菌（C. albicans）阴道感染的能力。该模型通过假发情小鼠阴道内接种酵母芽生孢子来重现。我们发现，感染后接受 salecan（每只小鼠 0.5mg）治疗的小鼠的 CFU 比接受生理盐水治疗的感染小鼠少 85%。与 C.albicans 组相比，salecan 减少了阴道中多形核白细胞（PMN）的迁移，降低了细胞因子 IL23、IL22、IL17a 和 IL17f、抗真菌基因 S100a8 和 S100a9 以及 C.albicans 模式识别受体 Dectin1 的 mRNA 水平。对不同分类水平阴道微生物群落组成的分析表明，salecan 处理组小鼠阴道中的细菌菌群组成与未感染小鼠相似，与感染小鼠不同。来自 salecan 处理组的阴道冲洗液中含有更多的肠球菌及其代谢产物乳酸。我们的结果表明，salecan 可能是治疗 C.albicans 阴道感染的潜在治疗剂。
