Molecular and Clinical Sciences Research Institute, St George's University of London, London, UK.
Molecular and Clinical Sciences Research Institute, St George's University of London, London, UK.
J Reprod Immunol. 2020 Apr;138:103082. doi: 10.1016/j.jri.2020.103082. Epub 2020 Jan 14.
During the first trimester of pregnancy the decidua is comprised of decidual stromal cells (DSC), invading fetal trophoblast cells and maternal leukocytes, including decidual natural killer (dNK) cells and macrophages. dNK cells are distinct from peripheral blood NK cells and have a role in regulating trophoblast invasion and spiral artery remodelling. The unique phenotype of dNK cells results from the decidual environment in which they reside, however the interaction and influence of other cells in the decidua on dNK phenotype is unknown. We isolated first trimester DSC and decidual macrophages and investigated the effect that DSC and decidual macrophage secreted factors have on CD56+ decidual lymphocyte receptor expression and cytokine secretion (including dNK cells). We report that DSC secreted factors induce the secretion of the cytokines IL-8 and IL-6 from first trimester CD56+ cells. However, neither DSC nor decidual macrophage secreted factors changed CD56+ cell receptor expression. These results suggest that secreted factors from DSC influence CD56+ decidual lymphocytes during the first trimester of pregnancy and therefore may play a role in regulating the unique phenotype and function of dNK cells during placentation.
在妊娠的头三个月，蜕膜由蜕膜基质细胞（DSC）组成，这些细胞会侵入胎儿滋养层细胞和母体白细胞，包括蜕膜自然杀伤（dNK）细胞和巨噬细胞。dNK 细胞与外周血 NK 细胞不同，在调节滋养层细胞侵袭和螺旋动脉重塑方面发挥作用。dNK 细胞的独特表型源于其所在的蜕膜环境，但目前尚不清楚蜕膜中的其他细胞与 dNK 表型的相互作用和影响。我们分离了妊娠早期的 DSC 和蜕膜巨噬细胞，并研究了 DSC 和蜕膜巨噬细胞分泌的因子对 CD56+ 蜕膜淋巴细胞受体表达和细胞因子分泌（包括 dNK 细胞）的影响。我们报告称，DSC 分泌的因子会诱导妊娠早期 CD56+ 细胞分泌细胞因子 IL-8 和 IL-6。然而，DSC 和蜕膜巨噬细胞分泌的因子均未改变 CD56+ 细胞的受体表达。这些结果表明，DSC 分泌的因子会在妊娠早期影响 CD56+ 蜕膜淋巴细胞，因此可能在调节胎盘形成过程中 dNK 细胞的独特表型和功能方面发挥作用。
