FAUNA Research Alliance and Conservation Biology Research Group, School of Environmental and Life Sciences, Biology Building, University of Newcastle, University Drive, Callaghan, NSW, 2308, Australia.
CSIRO Health and Biosecurity, Canberra, ACT, Australia.
Theriogenology. 2020 Mar 15;145:24-30. doi: 10.1016/j.theriogenology.2020.01.031. Epub 2020 Jan 16.
We investigated the capacity for pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (hCG) to induce synchronous oestrus and ovulation in the tammar wallaby (Notamacropus eugenii) after follicular suppression with Lucrin® Depot, a one-month GnRH agonist. On Day 0 pouch young were removed (RPY) to reactivate a normal oestrous cycle and then two groups were treated with vehicle (Control; n = 5 and Superovulation (SOvn); n = 5) and two groups were treated with 7.5 mg of Lucrin Depot (Lucrin; n = 6; Lucrin+SOvn, n = 6). On Day 20 RPY the SOvn and Lucrin+SOvn Group received 20 IU of PMSG, which was followed on Day 23 RPY with 500 IU of hCG. The Lucrin+SOvn females underwent a more synchronous oestrus with 5 of 6 mating on Day 26 RPY while the SOvn (n = 5/5) and Control (n = 5/5) Groups copulated over two days, between Day 25-27 RPY and Day 27-29 RPY respectively. Mating plugs were not detected in any of the females in the Lucrin Group by Day 31 RPY. Autopsy on Day 31 RPY confirmed all females in each treatment group had undergone a reactivated cycle as evidenced by the presence of a large corpus luteum (CL) in one ovary. At autopsy the females in the Lucrin+SOvn Group had highly stimulated reproductive tracts, and their ovaries contained many follicles >3 mm; 14 ± 2.1 and 15.3 ± 2.1 follicles >3 mm in the CL-bearing ovary and contralateral ovary respectively. Similarly, females in the SOvn Group had 11.4 ± 2.4 and 17.4 ± 1.9 follicles >3 mm in each respective ovary. Uterine flushing and ovarian histology confirmed that females in Lucrin+SOvn and SOvn Groups had not ovulated, but normal oocytes were present in the follicles. By comparison, the Control Group had ovulated with a single embryo being recovered from the uterus of 4 of 5 females. In contrast to all groups, females in the Lucrin Group showed follicular suppression (all follicles <1.5 mm) and an unstimulated reproductive tract. We conclude that a suppression plus stimulation regimen using Lucrin Depot followed by PMSG and hCG has the capacity to synchronise oestrus, and that 20 IU of PMSG stimulates the development of antral follicles >3 mm in both ovaries. However, a single 500 IU treatment of hCG on Day 23 RPY was not able to induce ovulation in the tammar wallaby.
我们研究了在 Lucrin® Depot（一种为期一个月的 GnRH 激动剂）抑制卵泡后，怀孕母马血清促性腺激素（PMSG）和人绒毛膜促性腺激素（hCG）诱导袋狸（Notamacropus eugenii）同步发情和排卵的能力。在第 0 天取出袋幼体（RPY）以重新激活正常发情周期，然后将两组用载体（对照组；n=5 和超数排卵（SOvn）；n=5）处理，两组用 7.5mg Lucrin Depot（Lucrin；n=6；Lucrin+SOvn，n=6）处理。在第 20 天 RPY 时，SOvn 和 Lucrin+SOvn 组接受 20IU 的 PMSG，然后在第 23 天 RPY 时接受 500IU 的 hCG。Lucrin+SOvn 组的发情更同步，有 6 只中的 5 只在第 26 天 RPY 交配，而 SOvn（n=5/5）和对照组（n=5/5）组分别在第 25-27 天 RPY 和第 27-29 天 RPY 之间交配两天。在第 31 天 RPY 时，Lucrin 组的任何雌性都没有检测到交配塞。第 31 天 RPY 的尸检证实，每个治疗组的所有雌性都经历了一个重新激活的周期，这表现为一个大的黄体（CL）在一个卵巢中。尸检时，Lucrin+SOvn 组的雌性生殖道受到高度刺激，她们的卵巢中有许多>3mm 的卵泡；CL 侧卵巢中有 14±2.1 个和 15.3±2.1 个>3mm 的卵泡，对侧卵巢分别有 11.4±2.4 个和 17.4±1.9 个>3mm 的卵泡。同样，SOvn 组的雌性在每个卵巢中分别有 11.4±2.4 个和 17.4±1.9 个>3mm 的卵泡。子宫冲洗和卵巢组织学证实，Lucrin+SOvn 和 SOvn 组的雌性没有排卵，但卵泡中存在正常的卵子。相比之下，对照组的 4 只雌性中有 5 只从子宫中回收了一个胚胎。与所有组相比，Lucrin 组的雌性表现出卵泡抑制（所有卵泡<1.5mm）和未受刺激的生殖道。我们得出结论，使用 Lucrin Depot 进行抑制加刺激方案，然后使用 PMSG 和 hCG，有能力同步发情，并且 20IU 的 PMSG 刺激两个卵巢中>3mm 的窦前卵泡的发育。然而，在第 23 天 RPY 时单次 500IU 的 hCG 处理不能诱导袋狸排卵。
