Future Industries Institute, University of South Australia, Mawson Lakes, South Australia 5095, Australia; School of Information Technology and Mathematical Sciences, University of South Australia, Mawson Lakes, South Australia 5095, Australia.
Future Industries Institute, University of South Australia, Mawson Lakes, South Australia 5095, Australia.
J Colloid Interface Sci. 2020 Apr 1;565:555-566. doi: 10.1016/j.jcis.2020.01.030. Epub 2020 Jan 15.
Polyelectrolyte multilayers composed of pharmaceutical grade fucoidan and chitosan have been assembled and studied in terms of their response to physiological solution conditions and the presence of lysozyme. The influence of phosphate buffered saline (PBS) solution on the multilayer was interrogated using attenuated total reflectance (ATR) Fourier transform infrared (FTIR) spectroscopy and atomic force microscopy (AFM). The combination of the techniques reveal that the polyelectrolyte multilayers swell when exposed to PBS after build-up and may include a small degree of mass loss as the film swells. The degree of swelling was influenced by the terminating layer of the multilayer. Upon exposure to lysozyme, it was observed that some deswelling occurred, as the enzyme adsorbed onto and permeated into the multilayer. The behaviour of the multilayer as a potential reservoir for lysozyme contrasts with the interaction with bovine serum albumin, which did not penetrate into the multilayer, indicating either exclusion by size or due to the overall net negative charge of the film.
由药用级褐藻胶和壳聚糖组成的聚电解质多层膜已被组装并进行了研究，以研究其对生理溶液条件和溶菌酶存在的响应。使用衰减全反射（ATR）傅里叶变换红外（FTIR）光谱和原子力显微镜（AFM）来研究磷酸盐缓冲盐水（PBS）溶液对多层膜的影响。这些技术的结合表明，在构建后暴露于 PBS 中时，聚电解质多层膜会发生溶胀，并且随着膜的膨胀可能会有少量的质量损失。溶胀的程度受多层膜的终止层的影响。当暴露于溶菌酶时，观察到一些溶胀发生，因为酶吸附到并渗透到多层膜中。作为溶菌酶潜在储库的多层膜的行为与与牛血清白蛋白的相互作用形成对比，牛血清白蛋白没有渗透到多层膜中，这表明要么是由于尺寸排除，要么是由于膜的总净负电荷。
