Zhang Shuaimin, Liu Yan, Liu Zhenhua, Zhang Yi, Chen Gen, Li Keyue, Tang Keli
Department of Hepatobiliary Surgery, Guizhou Provincial People's Hospital, Guiyang, China.
J BUON. 2019 Nov-Dec;24(6):2402-2410.
To investigate the expression level of circ_0000502 in hepatocellular carcinoma (HCC), and to further explore whether it can promote the malignant progression of HCC by targeting and binding to microRNA (miR)-124.
Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression level of circ_0000502 in 40 pairs of HCC tissue specimens and adjacent ones, and to analyze the relationship between circ_0000502 expression and prognosis of patients with HCC. QRT-PCR was used to verify the expression of circ_0000502 in HCC cells. The circ_0000502 knockdown model was constructed using lentivirus in HCC cell lines, and cell counting KIT-8 (CCK-8), Transwell and flow cytometry assays were used to figure out the effect of circ_0000502 on the function of HCC cells. Lastly, luciferase reporter gene assay was applied to verify the relationship between circ_0000502 and miR-124.
QRT-PCR results indicated that the level of circ_0000502 in HCC tissues was significantly higher than that in adjacent ones. Compared with patients with low expression of circ_0000502, patients with high expression of circ_0000502 had a lower overall survival rate compared with the negative control (NC) group. The proliferation, invasion and migration ability of circ_0000502 knockdown group significantly decreased, while on the contrary cell apoptosis increased. QRT-PCR results revealed that the expression of miR-124 and circ_0000502 mRNA in HCC tissues was negatively correlated. Also, the result of luciferase reporter gene assay demonstrated that circ_0000502 could be targeted by miR-124 via this binding site.
High expression of circ_0000502 was significantly positively correlated with poor prognosis of HCC. Besides, circ_0000502 promoted the malignant progression of HCC by regulating miR-124 expression.
研究环状RNA_0000502（circ_0000502）在肝细胞癌（HCC）中的表达水平，并进一步探讨其是否通过靶向结合微小RNA（miR）-124促进HCC的恶性进展。
采用定量实时聚合酶链反应（qRT-PCR）检测40对HCC组织标本及其癌旁组织中circ_0000502的表达水平，并分析circ_0000502表达与HCC患者预后的关系。用qRT-PCR验证circ_0000502在HCC细胞中的表达。在HCC细胞系中利用慢病毒构建circ_0000502敲低模型，采用细胞计数试剂盒-8（CCK-8）、Transwell和流式细胞术检测circ_0000502对HCC细胞功能的影响。最后，应用荧光素酶报告基因实验验证circ_0000502与miR-124的关系。
qRT-PCR结果显示，HCC组织中circ_0000502水平显著高于癌旁组织。与circ_0000502低表达患者相比，circ_0000502高表达患者的总生存率较低。circ_0000502敲低组的增殖、侵袭和迁移能力显著降低，而细胞凋亡增加。qRT-PCR结果显示，HCC组织中miR-124与circ_0000502 mRNA的表达呈负相关。此外，荧光素酶报告基因实验结果表明，circ_0000502可通过该结合位点被miR-124靶向。
circ_0000502高表达与HCC患者预后不良显著正相关。此外，circ_0000502通过调节miR-124表达促进HCC的恶性进展。
