Chen Junyi, Wang Fubo, Xu Huan, Chen Dong, Liu Weihui, Wang Jialiang
Department of Urology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou, China.
J BUON. 2019 Nov-Dec;24(6):2531-2538.
To explore the effects of Transmembrane-4-L-six-family-1 (TM4SF1) in prostate cancer (PCa), and the related underlying mechanisms.
PCa tissues were obtained from 78 patients. PCa cell lines DU145 and RWPE-2 were purchased from American Type Culture Collection (ATCC). Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were conducted to analyze the expression of TM4SF1 in PCa tissues and DU145 cells. Plasmid containing over-expressed TM4SF1 was achieved by plasmid transfection. Transwell assay and wound-healing assay were designed to examine the invasion and migration of DU145 cells, whereas colony formation assay and 5-Ethynyl-2'- deoxyuridine (EdU) staining assay were performed to study the proliferation ability of DU145 cells.
TM4SF1 was found over-expressed in PCa tissues and DU145 cells. Over-expression of TM4SF1 significantly activated the extracellular regulated protein kinases (ERK)1/2 signaling pathway, increased the epithelial-mesenchymal transition (EMT) expression, and enhanced the invasion, migration and proliferation of DU145 cells. Further studies revealed that suppression of ERK1/2 signaling pathway nearly resisted the positive effects on DU145 cells induced by TM4SF1 over-expression.
The present study demonstrated that TM4SF1 enhanced the invasion, migration and proliferation of DU145 cells by activating ERK1/2 signaling pathway.
探讨跨膜4-L六家族成员1（TM4SF1）在前列腺癌（PCa）中的作用及其相关潜在机制。
从78例患者中获取PCa组织。PCa细胞系DU145和RWPE-2购自美国典型培养物保藏中心（ATCC）。采用定量实时聚合酶链反应（qRT-PCR）和蛋白质免疫印迹法分析PCa组织和DU145细胞中TM4SF1的表达。通过质粒转染构建含过表达TM4SF1的质粒。设计Transwell实验和伤口愈合实验检测DU145细胞的侵袭和迁移能力，同时进行集落形成实验和5-乙炔基-2'-脱氧尿苷（EdU）染色实验研究DU145细胞的增殖能力。
发现TM4SF1在PCa组织和DU145细胞中过表达。TM4SF1的过表达显著激活细胞外调节蛋白激酶（ERK）1/2信号通路，增加上皮-间质转化（EMT）表达，并增强DU145细胞的侵袭、迁移和增殖能力。进一步研究表明，抑制ERK1/2信号通路几乎可抵抗TM4SF1过表达对DU145细胞的积极作用。
本研究表明，TM4SF1通过激活ERK1/2信号通路增强DU145细胞的侵袭、迁移和增殖能力。
