Mohammed Asmaa M, Hussen Dalia F, Rashad Hend, Hasheesh Adel
Department of Environmental and Occupational Medicine, National Research Centre, Doki, Egypt.
Department of Human Cytogenetics, National Research Centre, Doki, Egypt.
Asian Pac J Cancer Prev. 2020 Jan 1;21(1):87-92. doi: 10.31557/APJCP.2020.21.1.87.
The main aim of this study is to evaluate the micronuclei scoring as a biomarker for early detection and screening of genotoxic effect of cigarette smoking in the peripheral blood T- lymphocytes.
A total number of eligible 148 individuals have participated in the study; 78 Current smokers and 70 never smokers. Cytokinesis-block micronucleus assay was performed for all the participants in the peripheral blood T-lymphocytes. Assessment of the smoking status of the participants was conducted through the detailed smoking history, Fagerström test for nicotine dependence (FTND) scoring, and determination of the urinary cotinine creatinine ratio (CCR).
A significantly higher frequency of micronuclei in the binucleated T-lymphocytes(BMNi) was identified in the smokers group as compared to the nonsmokers; OR=4.9, 95% CI=1.9-12.5), P-value=0.006. Both of the pack years and the smoking duration of the smokers could significantly predict the BMNi scoring; P-value=0.001, 0.002 respectively.
Our results indicate the association between BMNi and cigarette smoking, suggesting that BMNi Scoring can be a useful biomarker for early detection and screening of the genotoxic effect of cigarette smoking as a primary preventive measure for various smoking induced cancers.
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本研究的主要目的是评估微核评分作为一种生物标志物，用于早期检测和筛查吸烟对外周血T淋巴细胞的遗传毒性作用。
共有148名符合条件的个体参与了本研究；78名当前吸烟者和70名从不吸烟者。对所有参与者的外周血T淋巴细胞进行了胞质分裂阻滞微核试验。通过详细的吸烟史、尼古丁依赖的Fagerström测试（FTND）评分以及尿可替宁肌酐比值（CCR）的测定来评估参与者的吸烟状况。
与不吸烟者相比，吸烟者组双核T淋巴细胞（BMNi）中的微核频率显著更高；比值比（OR）=4.9，95%置信区间（CI）=1.9-12.5），P值=0.006。吸烟者的吸烟包年数和吸烟持续时间均能显著预测BMNi评分；P值分别为0.001和0.002。
我们的结果表明BMNi与吸烟之间存在关联，提示BMNi评分可作为一种有用的生物标志物，用于早期检测和筛查吸烟的遗传毒性作用，作为预防各种吸烟诱发癌症的主要预防措施。
