Department of Clinical Veterinary Sciences, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
Faculty of Veterinary and Animal Sciences, Gono University, Savar 1344, Dhaka, Bangladesh.
J Reprod Dev. 2020 Apr 10;66(2):193-197. doi: 10.1262/jrd.2019-117. Epub 2020 Jan 26.
Until now, there have been no reports of foals born through embryo transfer after artificial insemination using frozen semen in Japan. The aims of this study were to develop a riding crossbred horse and evaluate the prospects of embryo transfer technology in multiplying horse population. In both donor and recipient mares, luteolysis was induced by the administration of 0.1 mg Cloprostenol to synchronize the onset of estrus, and ovulation was induced by administering 2000 IU human chorionic gonadotropin (hCG) or 0.75 mg Deslorelin. Frozen semen from an Irish Connemara pony stallion was used to breed a Hokkaido native pony mare by deep-horn artificial insemination (dose, 400 × 10 sperm). A non-surgical technique was used to collect embryos from the donor mare at day 7 post-ovulation and transfer them transcervically into the uterus of recipient mares (n = 4) immediately after collection. Weekly blood samples were collected from the recipients throughout pregnancy. A total of four embryos were recovered from seven collection attempts (57% recovery) from a donor mare in a single breeding season. Three of the four transferred embryos maintained successful pregnancy and delivered a healthy live foal (75% birth). A normal progesterone profile was observed throughout gestation in recipient mares. In conclusion, for the first time, to the best of our knowledge, this study describes the birth of foals through non-surgical transcervical embryo transfer in Japan after artificial insemination using frozen semen. We expect that this new crossbreed (Connemara pony × Hokkaido native pony) will be a good riding breed.
截至目前，在日本，尚无使用冷冻精液进行人工授精后通过胚胎移植产下驹的报道。本研究的目的是培育骑乘用杂交马，并评估胚胎移植技术在增加马种群数量方面的前景。在供体和受体母马中，通过注射 0.1mg 氯前列烯醇诱导黄体溶解，以同步发情，并通过注射 2000IU 人绒毛膜促性腺激素（hCG）或 0.75mg 地洛瑞林诱导排卵。使用爱尔兰康尼马拉种公马的冷冻精液通过深角人工授精（剂量为 400×10 精子）繁殖北海道本地母马。采用非手术技术从供体母马排卵后第 7 天收集胚胎，并在收集后立即经颈管将其移植到受体母马的子宫中（n=4）。在整个妊娠期间，每周从受体采集一次血样。在一个繁殖季节中，从一头供体母马的 7 次采集尝试中总共回收了 4 个胚胎（57%的回收率）。4 个移植胚胎中有 3 个成功维持妊娠并产下了健康的活驹（75%的出生率）。在受体母马中观察到整个妊娠期间孕激素水平正常。总之，据我们所知，这是首次在日本成功通过使用冷冻精液进行人工授精后的非手术经颈管胚胎移植产下驹。我们期望这种新的杂交种（康尼马拉种×北海道本地种）将成为一种优秀的骑乘品种。
