Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN), D-18196 Dummerstorf, Germany.
Institute of Behavioural Physiology, Leibniz Institute for Farm Animal Biology (FBN), D-18196 Dummerstorf, Germany.
J Reprod Dev. 2020 Apr 10;66(2):181-188. doi: 10.1262/jrd.2019-107. Epub 2020 Jan 27.
The aim of this study was to establish a model to induce cystic ovarian follicles (COFs) in cattle using the cyclooxygenase inhibitor, indomethacin. Eighteen Holstein-Frisian cattle were synchronized with prostaglandin F2alpha (PGF2α) and gonadotropin-releasing hormone (GnRH). Ultrasound-guided transvaginal intrafollicular injections were performed in 23 preovulatory follicles with different concentrations of indomethacin 16 h after GnRH administration. An injection of 0.2 ml 35 µM indomethacin solution (resulting in a final concentration of 8 µg/ml in the follicular fluid) was the minimal dosage leading to COF formation. The induced COFs reached a maximum mean diameter of 36.9 ± 4.5 mm eleven days after injection. The estrous cycle was extended to 25-39 days. Luteinization was first observed 4 days after injection, accompanied by a slight increase in plasma progesterone concentration. The bioactivity of indomethacin was demonstrated by the decrease of prostaglandin E in the follicular fluid of three animals. The method presented here is minimally invasive and allows for the generation of defined COFs for further investigations.
本研究旨在建立一种使用环氧化酶抑制剂吲哚美辛诱导牛囊性卵巢卵泡（COF）的模型。18 头荷斯坦弗里森奶牛通过前列腺素 F2α（PGF2α）和促性腺激素释放激素（GnRH）同步化。在 GnRH 给药后 16 小时，对 23 个发情前卵泡进行了超声引导下的经阴道腔内卵泡内注射，注射了不同浓度的吲哚美辛 0.2ml35µM 吲哚美辛溶液（导致卵泡液中的终浓度为 8µg/ml）是导致 COF 形成的最小剂量。诱导的 COF 在注射后 11 天达到最大平均直径 36.9±4.5mm。发情周期延长至 25-39 天。注射后 4 天首次观察到黄体化，同时血浆孕酮浓度略有升高。通过在三个动物的卵泡液中减少前列腺素 E，证明了吲哚美辛的生物活性。本文提出的方法微创且可以产生特定的 COF 以进行进一步研究。
