Phelps C J, Wiggins J P, Wangsness P J
Pennsylvania State University, University Park 16802.
J Anim Sci. 1988 Oct;66(10):2614-25. doi: 10.2527/jas1988.66102614x.
A series of experiments was conducted to evaluate the effect of zeranol on release and synthesis of growth hormone (GH) by anterior pituitary cells established in either static or continuous flow cultures. Young adult male rats, slaughter-age lambs and juvenile lambs were used as sources of pituitary cells. In static primary cell cultures, no consistent effect of zeranol at 10(-7), 10(-9) or 10(-11) M was demonstrated by either rat or ovine cells. Rat pituitaries established in perifusion culture chambers showed no repeatable response to zeranol. Dissociated cells from lambs established in perifusion culture, however, had significant increases in release of GH in response to 37% of zeranol pulse exposures. When dissociated cells from juvenile lamb pituitaries were used, up to 10-fold increases in GH release consistently were measured within minutes of exposure to zeranol.
进行了一系列实验，以评估玉米赤霉醇对在静态或连续流动培养中建立的垂体前叶细胞释放和合成生长激素（GH）的影响。使用成年雄性幼鼠、屠宰期羔羊和幼年羔羊作为垂体细胞的来源。在静态原代细胞培养中，无论是大鼠还是绵羊细胞，均未证明10^(-7)、10^(-9)或10^(-11) M的玉米赤霉醇具有一致的作用。在灌注培养室中建立的大鼠垂体对玉米赤霉醇没有可重复的反应。然而，在灌注培养中建立的羔羊解离细胞，对37%的玉米赤霉醇脉冲暴露有显著的GH释放增加。当使用幼年羔羊垂体的解离细胞时，在暴露于玉米赤霉醇的几分钟内，GH释放持续测量到高达10倍的增加。
