脂肪来源干细胞在辐照软骨上的软骨生成。

Chondrogenesis of Adipose-Derived Stem Cells on Irradiated Cartilage.

机构信息

From the Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine; and the Department of Plastic and Reconstructive Surgery, Dongguk University Medical Center.

出版信息

Plast Reconstr Surg. 2020 Feb;145(2):409-418. doi: 10.1097/PRS.0000000000006482.

DOI:10.1097/PRS.0000000000006482

PMID:31985633

Abstract

BACKGROUND

Irradiated allogeneic costal cartilage is an alternative option of cartilage graft in patients with insufficient autologous cartilage. However, complications can occur during long-term follow-up. This study investigated whether Tutoplast-processed cartilage, one of the irradiated allogeneic costal cartilages, acts as a scaffold for adipose-derived stem cells and chondrogenesis.

METHODS

In vitro setting, human adipose-derived stem cells seeded onto Tutoplast-processed cartilage were cultured in chondrogenic medium and observed using a scanning electron microscope. Next, 3 types of irradiated cartilage-including Tutoplast-processed cartilage, undifferentiated stem cells on Tutoplast-processed cartilage (undifferentiated group), and chondrogenic differentiated stem cells on Tutoplast-processed cartilage (chondrogenic group)-were implanted subcutaneously into nude mice. Gross, histologic, and gene expression analyses of Tutoplast-processed cartilages were performed at postoperative weeks 2 and 4.

RESULTS

Human adipose-derived stem cells subjected to in vitro three-dimensional culture differentiated into chondrocytes and expressed cartilage-specificgenes. Adipose-derived stem cells seeded onto Tutoplast-processed cartilage were differentiated into chondrocytes in chondrogenic medium. In the chondrogenic group, the chondrogenic-differentiated cells attached to the surface of the Tutoplast-processed cartilage were maintained during the follow-up and were distinct from the existing Tutoplast-processed cartilage. Moreover, the chondrogenic group had higher expression of cartilage-specific genes compared with the undifferentiated group.

CONCLUSIONS

Adipose-derived stem cells seeded onto Tutoplast-processed cartilage underwent chondrogenic differentiation, generating new cartilage, which was maintained after implantation without critical complications. The findings are clinically valuable in terms of overcoming the limitations of irradiated allogeneic costal cartilage, and broaden the surgical options for treatments requiring cartilage.

摘要

背景

在自体软骨量不足的患者中，辐照同种异体肋软骨是软骨移植物的一种替代选择。然而，在长期随访中可能会发生并发症。本研究旨在探讨经 Tutoplast 处理的软骨（一种辐照同种异体肋软骨）是否可作为脂肪来源干细胞和成软骨的支架。

方法

在体外环境中，将人脂肪来源干细胞接种到经 Tutoplast 处理的软骨上，在软骨形成培养基中培养，并使用扫描电子显微镜进行观察。接下来，将 3 种辐照软骨（包括经 Tutoplast 处理的软骨、经 Tutoplast 处理的软骨上未分化的干细胞[未分化组]和经 Tutoplast 处理的软骨上分化的软骨细胞[成软骨组]）皮下植入裸鼠。在术后 2 周和 4 周时，对经 Tutoplast 处理的软骨进行大体观察、组织学和基因表达分析。

结果

体外三维培养的人脂肪来源干细胞分化为软骨细胞，并表达软骨特异性基因。在软骨形成培养基中，接种到经 Tutoplast 处理的软骨上的脂肪来源干细胞分化为软骨细胞。在成软骨组中，附着在经 Tutoplast 处理的软骨表面的成软骨分化细胞在随访期间得以维持，且与现有的经 Tutoplast 处理的软骨不同。此外，与未分化组相比，成软骨组的软骨特异性基因表达更高。