Agriculture and Agri-Food Canada, 5403-1st Avenue South, Lethbridge, AB T1J 4B1, Canada.
Department of Agricultural, Food and Nutritional Science, 410 Agriculture/Forestry Centre, University of Alberta, Edmonton, AB T6G 2P5, Canada.
Can J Microbiol. 2020 Apr;66(4):288-302. doi: 10.1139/cjm-2019-0469. Epub 2020 Jan 27.
Characterization of the microbiota of chickens is of current interest. The goals of the current study were to apply anaerobic isolation methods to comprehensively isolate and identify bacteria from the gastrointestinal tract of chickens and their environment. Bacterial communities within the drinking water were dominated by , whereas communities in litter were more representative of the cecum. The crop and small intestine (jejunum and ileum) were dominated by and spp., and the cecum was dominated by spp. The collection of bacteria isolated was dominated by spp., / spp., spp., and spp.; however, many rare taxa were observed. These included members of the and spp., which were commonly isolated from the ileum and cecum. Bacteria isolated by enrichment and direct plating differed. The selective de Man-Rogosa-Sharpe agar was commonly associated with the isolation of spp. and yielded the lowest diversity of all methods utilized. Increased diversity and frequency of spp. was observed in enrichments of blood and mucus or by plating on Columbia agar supplemented with 10% blood and gentamicin. The bacteria isolated from this study provide source material for genomic and functional studies in chicken hosts.
对鸡的微生物群落进行特征分析是当前的研究热点。本研究旨在采用厌氧分离方法，全面分离和鉴定鸡及其环境中的肠道细菌。饮用水中的细菌群落主要由 菌属主导，而垫料中的群落则更能代表盲肠。鸡的嗉囊和小肠（空肠和回肠）主要由 菌属和 菌属主导，而盲肠则主要由 菌属主导。所分离的细菌主要由 菌属、/菌属、 菌属和 菌属主导；然而，也观察到了许多稀有类群。其中包括常见于空肠和盲肠的 菌属和 菌属成员。通过富集和直接平板培养分离出的细菌有所不同。选择性的脑心浸液琼脂通常与 菌属的分离有关，其产生的多样性是所有使用方法中最低的。在血液和黏液的富集物或在添加 10%血液和庆大霉素的哥伦比亚琼脂平板上进行平板培养时，观察到 菌属的多样性和丰度增加。本研究中分离出的细菌为鸡宿主的基因组和功能研究提供了材料来源。
