School of Food Science and Engineering, South China University of Technology, 381Wushan Road, Guangzhou 510641, P.R. China.
Innovation Center of Bioactive Molecule Development and Application, South China Institute of Collaborative Innovation, Xuefu Road, Dongguan 221116, P.R. China.
J Microbiol Biotechnol. 2020 Apr 28;30(4):561-570. doi: 10.4014/jmb.1910.10043.
This study was designed to synthesize triglycerol monolaurate (TGML) with Lipozyme 435 as the catalyst, and explore its effects on the growth of () and () and the secretion of aflatoxin b1. The highest content of TGML (49.76%) was obtained at a molar ratio of triglycerol to lauric acid of 1.08, a reaction temperature of 84.93°C, a reaction time of 6 h and an enzyme dosage of 1.32%. After purification by molecular distillation combined with the washes with ethyl acetate and water, the purity of TGML reached 98.3%. Through characterization by electrospray-ionization mass spectrometry, infrared spectrum and nuclear magnetic resonance, the structure of TGML was identified as a linear triglycerol combined with lauroyl at the end. Finally, the inhibitory effects of TGML on the growths of and and the secretion of aflatoxin b1 were evaluated by measuring the colony diameter, the inhibition rate of mycelial growth and the content of mycotoxin in the media. The results indicated that TGML had a stronger inhibitory effects on colony growth and mycelial development of both toxic molds compared to sodium benzoate and potassium sorbate, and the secretions of toxins from and were completely suppressed when adding TGML at 10 and 5 mM, respectively. Based on the above results, TGML may be used as a substitute for traditional antifungal agents in the food industry.
本研究旨在以脂肪酶 435 为催化剂合成三甘醇单月桂酸酯（TGML），并探讨其对（）和（）生长和黄曲霉毒素 b1 分泌的影响。在三甘醇与月桂酸摩尔比为 1.08、反应温度为 84.93°C、反应时间为 6 h 和酶用量为 1.32%的条件下，TGML 的含量最高（49.76%）。经分子蒸馏与乙酸乙酯和水洗涤相结合纯化后，TGML 的纯度达到 98.3%。通过电喷雾电离质谱、红外光谱和核磁共振对 TGML 的结构进行表征，鉴定其为线性三甘醇与脂肪酰基末端结合。最后，通过测量平板上的菌落直径、菌丝生长抑制率和培养基中真菌毒素含量，评价了 TGML 对两种产毒真菌生长和黄曲霉毒素 b1 分泌的抑制作用。结果表明，与苯甲酸钠和山梨酸钾相比，TGML 对两种产毒真菌的菌落生长和菌丝发育具有更强的抑制作用，当添加浓度分别为 10 和 5 mM 的 TGML 时，毒素的分泌完全受到抑制。基于上述结果，TGML 可能在食品工业中用作传统抗真菌剂的替代品。
