Enders G C, Millette C F
Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, MA 02115.
J Cell Sci. 1988 May;90 ( Pt 1):105-14. doi: 10.1242/jcs.90.1.105.
Spermatogenic cells differentiate in vivo while in continuous contact with the Sertoli cell. During differentiation, the spermatogenic cells and Sertoli cells form a number of morphologically distinct stage-specific adhesions. We describe an in vitro assay system for studying the adhesion of spermatogenic cells to Sertoli cell monolayers. Mixed populations of spermatogenic cells or enriched fractions of pachytene spermatocytes and round spermatids were labelled with the vital dye, fluorescein diacetate, prior to their addition to Sertoli cell monolayers so that the adhesion of viable spermatogenic cells could be quantified. Using this assay system, the number of pachytene spermatocyte and round spermatid binding sites on the Sertoli cell monolayer were similar, but the kinetics of binding were different. Pachytene spermatocytes were able to inhibit significantly round spermatid binding, while round spermatids did not significantly inhibit pachytene spermatocyte binding. After coculture for 24-48 h, spermatocytes form junctional structures with Sertoli cells that are similar to desmosome-like junctions. These results suggest that pachytene spermatocytes and round spermatids bind to Sertoli cells by different mechanisms.
生精细胞在体内分化时始终与支持细胞保持接触。在分化过程中，生精细胞与支持细胞形成许多形态上不同的阶段特异性黏附。我们描述了一种用于研究生精细胞与支持细胞单层黏附的体外检测系统。在将生精细胞混合群体或粗线期精母细胞和圆形精子细胞富集组分添加到支持细胞单层之前，先用活性染料荧光素二乙酸酯对其进行标记，以便能够对活的生精细胞的黏附进行定量。使用该检测系统，支持细胞单层上粗线期精母细胞和圆形精子细胞的结合位点数量相似，但结合动力学不同。粗线期精母细胞能够显著抑制圆形精子细胞的结合，而圆形精子细胞对粗线期精母细胞的结合没有显著抑制作用。共培养24 - 48小时后，精母细胞与支持细胞形成类似于桥粒样连接的连接结构。这些结果表明，粗线期精母细胞和圆形精子细胞通过不同机制与支持细胞结合。
