Faculty of Mathematics and Natural Sciences, Laboratory of Bioelectromagnetism, University of Rzeszow, Rzeszow, Poland.
Institute of Nuclear Physics Polish Academy of Sciences, Krakow, Poland.
Anim Reprod Sci. 2020 Feb;213:106258. doi: 10.1016/j.anireprosci.2019.106258. Epub 2019 Dec 14.
Electromagnetic fields (EMF) are classified as an environmental factor affecting living organisms. The aim of the study was to evaluate the effect of EMF at different frequencies (50 and 120 Hz), durations of treatment (2 and 4 h) and with the magnetic induction of 8 m T on testicular tissues of roe deer (Capreolus capreolus) in vitro by comparison with the control samples. Fourier Transform Raman Spectroscopy (FT-Raman) and Fourier Transform Infrared Spectroscopy (FTIR) were utilized in this study to identify the chemical changes in the testicular tissues. The FTIR and FT-Raman spectroscopy methods were used to evaluate differences in spectra of the treated tissues compared to the control group. The results from the analysis of the spectra indicated there were characteristic differences in the testicular tissue compared with the control samples. There was identification of peaks attributed to different biochemical components. Comparing the spectra for different frequencies and treatment times, there was a greater intensity of peaks originating from most of the functional groups in the tissues evaluated. With the FTIR spectra, there were five of 15 peaks, while with the FT-Raman spectra, there were six of ten peaks that were shifted. For FTIR and FT-Raman analyzed spectral ranges, results from the PCA analysis indicate there was no similarity between control groups (2 and 4 h) and samples treated with EMF at a frequency of 120 Hz for 2 and 4 h. In conclusion, therefore, EMF is an environmental factor affecting the testis of roe deer.
电磁场（EMF）被归类为影响生物的环境因素。本研究的目的是通过与对照组比较，评估不同频率（50 和 120 Hz）、治疗时间（2 和 4 小时）和磁场强度为 8 mT 的 EMF 对体外狍睾丸组织的影响。傅里叶变换拉曼光谱（FT-Raman）和傅里叶变换红外光谱（FTIR）用于鉴定睾丸组织的化学变化。FTIR 和 FT-Raman 光谱方法用于评估处理组织与对照组之间的光谱差异。对光谱的分析结果表明，与对照组相比，睾丸组织存在特征差异。鉴定出归因于不同生化成分的峰。比较不同频率和治疗时间的光谱，评估的组织中大多数功能基团的起源峰强度更大。FTIR 光谱中有 15 个峰中的 5 个，而 FT-Raman 光谱中有 10 个峰中的 6 个发生了位移。对于 FTIR 和 FT-Raman 分析的光谱范围，PCA 分析的结果表明，对照组（2 和 4 小时）与在 120 Hz 频率下处理 2 和 4 小时的 EMF 样品之间没有相似性。因此，综上所述，电磁场是影响狍睾丸的环境因素。
