Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, China.
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, China.
Anim Reprod Sci. 2020 Feb;213:106276. doi: 10.1016/j.anireprosci.2020.106276. Epub 2020 Jan 8.
There is production of prostaglandin F (PGF) and there is PGF receptor (PTGFR) mRNA transcript in endometrial epithelial cells of cattle. The aims of the present study were to (1) determine whether PGF-PTGFR signaling modulates the proliferation of endometrial epithelial cells and (2) increase knowledge of PGF-PTGFR signaling on the physiological and pharmacological processes in the endometrium of cattle. Amount of cellular proliferation was determined using real-time cell analysis and cell proliferation reagent WST-1 procedures. Abundance of cyclins, cyclin-dependent kinases (CDKs), cyclin-kinase inhibitors, proliferating cell nuclear antigen (PCNA), cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), PTGFR, epidermal growth factor (EGF) mRNA and protein abundances were evaluated using real-time RT-PCR and western blot analyses. The PGF-PTGFR signaling promoted the proliferation of endometrial epithelial cells by inducing changes in abundance of mRNA transcript and protein that resulted in an increase in the abundance for the cyclins (A, B1, D1, D3), CDKs (1, 2, 4, 6), and PCNA; decrease in abundance for p21; and increase in abundance for EGF, COX-1, COX-2, and PTGFR. There was a direct molecular association between PGF-PTGFR signaling and cell cycle regulation in endometrial epithelial cells of cattle. In addition, findings improve the understanding of PGF-PTGFR signaling in the physiological and pharmacological processes of the endometrium of cattle.
牛子宫内膜上皮细胞中存在前列腺素 F（PGF）的产生和 PGF 受体（PTGFR）mRNA 转录物。本研究的目的是：（1）确定 PGF-PTGFR 信号是否调节子宫内膜上皮细胞的增殖；（2）增加对 PGF-PTGFR 信号在牛子宫内膜的生理和药理过程中的认识。使用实时细胞分析和细胞增殖试剂 WST-1 程序来确定细胞增殖的量。使用实时 RT-PCR 和 Western blot 分析评估细胞周期蛋白、细胞周期蛋白依赖性激酶（CDK）、细胞周期蛋白激酶抑制剂、增殖细胞核抗原（PCNA）、环氧化酶-1（COX-1）、环氧化酶-2（COX-2）、PTGFR、表皮生长因子（EGF）mRNA 和蛋白的丰度。PGF-PTGFR 信号通过诱导 mRNA 转录物和蛋白丰度的变化来促进子宫内膜上皮细胞的增殖，从而增加细胞周期蛋白（A、B1、D1、D3）、CDK（1、2、4、6）和 PCNA 的丰度；降低 p21 的丰度；增加 EGF、COX-1、COX-2 和 PTGFR 的丰度。在牛子宫内膜上皮细胞中，PGF-PTGFR 信号与细胞周期调控之间存在直接的分子关联。此外，这些发现提高了对 PGF-PTGFR 信号在牛子宫内膜生理和药理过程中的认识。
