HASPIN 参与了胆囊癌的进展。
HASPIN is involved in the progression of gallbladder carcinoma.
机构信息
Department of Gynaecology and Obstetrics, Daping Hospital, Army Medical University, China.
Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, China.
出版信息
Exp Cell Res. 2020 May 15;390(2):111863. doi: 10.1016/j.yexcr.2020.111863. Epub 2020 Jan 25.
BACKGROUND
Gallbladder carcinoma (GBC) is a common malignant tumor of the biliary system, but the current treatment of GBC is unsatisfactory. Therefore, new treatment targets and strategies are urgently needed.
METHODS
The expression of HASPIN in GBC was detected by immunohistochemical staining. HASPIN knockdown cell model was constructed by lentivirus infection, and the infection efficiency of lentivirus and knockdown efficiency of shHASPIN were verified by fluorescence immunoassay, qRT-PCR and Western blot. The effects of HASPIN knockdown on cell proliferation, clone-formation ability and apoptosis were determined by MTT, clone formation assay, flow cytometry and Human Apoptosis Antibody Array in vitro. Besides, the effect of HASPIN knockdown on the growth of GBC solid tumors was demonstrated in vivo.
RESULTS
The expression of HASPIN in GBC was up-regulated and positively correlated with the pathological grade of GBC. ShHASPIN significantly down-regulated the mRNA and protein levels of HASPIN, suggesting that HASPIN knockdown cell model was successfully constructed in vitro. After HASPIN knockdown, the proliferation and clone-formation ability of GBC cells were observably inhibited, the apoptotic levels were markedly increased, and the expression of Caspase 3, IGFBP-5, p21 and sTNF-R1 related to apoptotic pathway was up-regulated. Furthermore, HASPIN knockdown inhibited the growth of GBC in vivo.
CONCLUSION
HASPIN was up-regulated in GBC and played an important role in promoting the progress of GBC.
背景
胆囊癌（GBC）是一种常见的胆道系统恶性肿瘤，但目前 GBC 的治疗效果并不令人满意。因此，迫切需要新的治疗靶点和策略。
方法
通过免疫组织化学染色检测 GBC 中 HASPIN 的表达。通过慢病毒感染构建 HASPIN 敲低细胞模型，通过荧光免疫检测、qRT-PCR 和 Western blot 验证慢病毒的感染效率和 shHASPIN 的敲低效率。体外通过 MTT、克隆形成实验、流式细胞术和人凋亡抗体阵列检测 HASPIN 敲低对细胞增殖、克隆形成能力和细胞凋亡的影响。此外，在体内证明了 HASPIN 敲低对 GBC 实体瘤生长的影响。
结果
HASPIN 在 GBC 中的表达上调，并与 GBC 的病理分级呈正相关。shHASPIN 显著下调 HASPIN 的 mRNA 和蛋白水平，表明体外成功构建了 HASPIN 敲低细胞模型。HASPIN 敲低后，GBC 细胞的增殖和克隆形成能力明显受到抑制，凋亡水平明显增加，与凋亡途径相关的 Caspase 3、IGFBP-5、p21 和 sTNF-R1 的表达上调。此外，HASPIN 敲低抑制了 GBC 在体内的生长。
结论
HASPIN 在 GBC 中上调，在促进 GBC 进展中发挥重要作用。
Zotero 插件