Institute of Modern Aquaculture Science and Engineering, School of Life Sciences, South China Normal University, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangdong Provincial Engineering Technology Research Center for Environmentally-Friendly Aquaculture, Guangzhou, 510631, PR China.
Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, PR China.
Dev Comp Immunol. 2020 May;106:103629. doi: 10.1016/j.dci.2020.103629. Epub 2020 Jan 24.
Interleukin 6 (IL-6) is a pleiotropic cytokine that exerts its biological functions through interaction with its receptor system consisting of a ligand-specific IL-6 receptor (IL-6R) and a common signal-transducing receptor (gp130). In this study, OnIL-6R and Ongp130 genes from Nile tilapia (Oreochromis niloticus) were identified, and their roles in bacterial or viral infection and in regulation of inflammatory response involved in IL-6 were investigated. The open reading frames (ORFs) of OnIL-6R and Ongp130 are 2019 bp and 2679 bp, encoding 672 and 892 amino acids, respectively. Domain analysis of the deduced amino acid sequences of OnIL-6R and Ongp130 showed that both of them contained a conserved Ig-like domain, FNIII domains, and a WSXWS motif. The transcripts of OnIL-6R and Ongp130 were widely expressed in all examined tissues. Following in vivo challenges with Streptococcus agalactia, Poly I: C and lipopolysaccharide (LPS), the mRNAs of OnIL-6R and Ongp130 were notably induced in liver, head kidney and spleen. The transcriptional up-regulations of OnIL-6R and Ongp130 were also detected in Nile tilapia monocytes/macrophages and lymphocytes after in vitro stimulations with S. agalactiae, Poly I: C and LPS. Besides, increasing mRNA levels of the inflammation-related cytokines (IL-1β, TNF-α, IL-6, IL-10, and MIF) induced by recombinant OnIL-6 could be further enhanced by co-treatment with recombinant soluble OnIL-6R in lymphocytes. Furthermore, recombinant soluble Ongp130 suppressed the induction of expression of these cytokines in lymphocytes when co-stimulated with (r)OnIL-6 and (r)sOnIL-6R. Taken together, these results indicated that OnIL-6R and Ongp130 were likely involved in the resistance to bacterial or viral infection in Nile tilapia. Moreover, soluble OnIL-6R and soluble Ongp130 have an agonistic effect or antagonistic effect in the inflammation response involved in OnIL-6.
白细胞介素 6（IL-6）是一种多效细胞因子，通过与其受体系统相互作用发挥其生物学功能，该受体系统由配体特异性的白细胞介素 6 受体（IL-6R）和共同的信号转导受体（gp130）组成。在这项研究中，鉴定了尼罗罗非鱼（Oreochromis niloticus）的 OnIL-6R 和 Ongp130 基因，并研究了它们在细菌或病毒感染以及白细胞介素 6 参与的炎症反应调节中的作用。OnIL-6R 和 ong p130 的开放阅读框（ORF）分别为 2019bp 和 2679bp，分别编码 672 和 892 个氨基酸。OnIL-6R 和 ong p130 推导的氨基酸序列的结构域分析表明，它们都包含一个保守的 Ig 样结构域、FNIII 结构域和 WSXWS 基序。OnIL-6R 和 ong p130 的转录本在所有检测到的组织中广泛表达。在体内受到无乳链球菌、聚 I：C 和脂多糖（LPS）的刺激后，肝、头肾和脾中 OnIL-6R 和 ong p130 的 mRNA 明显诱导。在体外用无乳链球菌、聚 I：C 和 LPS 刺激尼罗罗非鱼单核细胞/巨噬细胞和淋巴细胞后，也检测到 OnIL-6R 和 ong p130 的转录上调。此外，重组可溶性 OnIL-6R 可进一步增强重组 OnIL-6 诱导的炎症相关细胞因子（IL-1β、TNF-α、IL-6、IL-10 和 MIF）mRNA 水平的增加。此外，当与（r）OnIL-6 和（r）sOnIL-6R 共刺激时，重组可溶性 ong p130 抑制了淋巴细胞中这些细胞因子表达的诱导。总之，这些结果表明，OnIL-6R 和 ong p130 可能参与了尼罗罗非鱼对细菌或病毒感染的抵抗。此外，可溶性 OnIL-6R 和可溶性 ong p130 在 OnIL-6 参与的炎症反应中具有激动剂或拮抗剂作用。
