Alzoubi Karem H, Al-Jamal Fatima F, Mahasneh Amjad F
Department of Clinical Pharmacy, Jordan University of Science and Technology, Irbid, 22110, Jordan.
Department of Applied Biology, Jordan University of Science and Technology, Irbid, Jordan.
Physiol Behav. 2020 Apr 1;217:112823. doi: 10.1016/j.physbeh.2020.112823. Epub 2020 Jan 25.
Sleep plays a critical role in body health maintenance and well-being throughout a lifetime. Sleeplessness is the most prevalent feature in the modernist societies, which impairs memory. Studies have shown a strong correlation between sleep deprivation and memory impairment in humans and animals, which is related to oxidative stress induced damage within the brain. Cerebrolysin (CBL) is a neuropeptide that is used to treat vascular dementia. In this study, the effect of CBL on sleep-deprivation induced memory impairment was investigated. Sleep deprivation was induced in rats using the modified multiple platform model (8 h/day for 4 weeks). Concurrently, CBL was administered intraperitoneally for four weeks. Animal's performance (spatial learning and memory) was assessed using the radial arm water maze (RAWM). Additionally, oxidative stress biomarkers including reduced glutathione (GSH), oxidized Glutathione (GSSG), GSH/GSSG ratio, glutathione peroxidase (GPx), and catalase were assessed in the hippocampus. Current results showed that sleep deprivation impaired both short- and long- term memories (P < 0.05), and that chronic CBL administration prevented such impairment. Additionally, CBL prevented decreases in hippocampal GPx, catalase, GSH and GSH/GSSG ratio and normalized increases in GSSG levels, which were impaired by the sleep deprivation model (P < 0.05). In conclusion, result showed a protective effect for CBL administration against sleep-deprivation induced memory impairment, which could be related to CBL induced prevention of oxidative stress in the hippocampus.
睡眠在人的一生中对维持身体健康和幸福起着至关重要的作用。失眠是现代社会最普遍的特征，它会损害记忆力。研究表明，人类和动物的睡眠剥夺与记忆损伤之间存在很强的相关性，这与大脑内氧化应激诱导的损伤有关。脑蛋白水解物（CBL）是一种用于治疗血管性痴呆的神经肽。在本研究中，研究了CBL对睡眠剥夺诱导的记忆损伤的影响。使用改良的多平台模型（每天8小时，持续4周）诱导大鼠睡眠剥夺。同时，腹腔注射CBL四周。使用放射状臂水迷宫（RAWM）评估动物的行为（空间学习和记忆）。此外，在海马体中评估了氧化应激生物标志物，包括还原型谷胱甘肽（GSH）、氧化型谷胱甘肽（GSSG）、GSH/GSSG比值、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶。目前的结果表明，睡眠剥夺损害了短期和长期记忆（P<0.05），而长期给予CBL可预防这种损害。此外，CBL可防止海马体中GPx、过氧化氢酶、GSH和GSH/GSSG比值的降低，并使睡眠剥夺模型所损害的GSSG水平升高恢复正常（P<0.05）。总之，结果表明给予CBL对睡眠剥夺诱导的记忆损伤具有保护作用，这可能与CBL诱导预防海马体中的氧化应激有关。
