利多卡因浓度对小梁切除术中人眼成纤维细胞生长的影响：一项体外研究。
Effect of Lignocaine Concentration on Human Fibroblasts Growth in Eyes Undergoing Trabeculectomy: An in vitro Study.
作者信息
Janani Madhuravasal Krishnan, Jaichandran Venkatakrishnan, Madhavan Hajib Narahari Rao, Vijaya Lingam, George Ronnie Jacob, Ambastha Priyam Shrikant, Manu Abhijeet, Malathi Jambulingam
机构信息
L&T Microbiology Research Centre, Vision Research Foundation, Chennai, India.
Department of Anaesthesiology, Vision Research Foundation, Sankara Nethralaya, Chennai, India.
出版信息
Biomed Hub. 2018 Oct 23;3(3):1-10. doi: 10.1159/000491074. eCollection 2018 Sep-Dec.
PURPOSE
To evaluate the effect of lignocaine on growth and apoptosis indication of primary human Tenon's capsule fibroblast (HTFs) in an in vitro model.
PATIENTS AND METHODS
Tenon's capsule tissue obtained from patients undergoing trabeculectomy were grown in cell culture medium. The effect of different concentrations of lignocaine (0.5, 1.0, 1.5, and 2%) on the morphology and growth of the fibroblasts was studied using microscopy, cell viability, and proliferation assay, and apoptosis was detected using the FITC Annexin V Apoptosis Kit.
RESULTS
Morphological changes similar to those of apoptotic cells, including cytoplasmic vacuolation, shrinkage, and rounding were visualized in the cells treated with concentrations greater than 1.0% (i.e., 1.5, 2.0%). Though proliferation inhibition was found with all four concentrations (0.5-2.0%), the viability of cells decreased from 1.0% lignocaine.
CONCLUSION
0.5% lignocaine prevents proliferation of fibroblasts without causing apoptosis in vitro.
目的
在体外模型中评估利多卡因对原代人眼球筋膜囊成纤维细胞（HTFs）生长和凋亡指标的影响。
患者与方法
从接受小梁切除术的患者获取眼球筋膜囊组织，在细胞培养基中培养。使用显微镜、细胞活力和增殖测定法研究不同浓度（0.5%、1.0%、1.5%和2%）利多卡因对成纤维细胞形态和生长的影响，并使用FITC膜联蛋白V凋亡试剂盒检测凋亡情况。
结果
在用浓度大于1.0%（即1.5%、2.0%）处理的细胞中可见到与凋亡细胞相似的形态学变化，包括细胞质空泡化、收缩和变圆。虽然所有四种浓度（0.5 - 2.0%）均发现有增殖抑制，但从1.0%利多卡因起细胞活力下降。
结论
0.5%利多卡因在体外可防止成纤维细胞增殖而不引起凋亡。
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