Flavell S U, Flavell D J
University Department of Haematology, Southampton General Hospital, Hampshire, U.K.
J Immunol Methods. 1988 Dec 9;115(2):179-85. doi: 10.1016/0022-1759(88)90286-4.
A mouse monoclonal antibody (NO-1) with specificity for the anti-cancer drug mitozantrone (MZ) (Novantrone) was produced by immunization of a BALB/c mouse with mitozantrone-keyhole limpet haemocyanin (MZ-KLH) conjugate. When used in an indirect competitive enzyme-linked immunosorbent assay (ELISA), NO-1 permitted the accurate and reproducible detection of between 0.25-50 ng/ml of MZ in pooled human serum, the standard curve obtained within this range being virtually linear. The assay demonstrated good reproducibility with intra-assay coefficients of variation (CV) of between 1.41% and 7.02% and an inter-assay CV of 3.45%. Regression analysis of levels of MZ detected by ELISA vs. the actual amounts added to pooled human serum gave a very good correlation coefficient of r = 0.995. NO-1 showed no cross-reactivity with either bisantrene or daunorubicin. A simple pharmacokinetic study was undertaken in rabbits given MZ intravenously at a dose of 0.5 mg/kg of body weight. Levels of MZ in rabbit serum measured with the assay ranged between 82 and 170 ng/ml for rabbits 1 and 2, respectively at 15 min falling to 1.25 ng/ml by 48 h for rabbit 1 and falling to undetectable levels by 120 h for rabbit 2.
用米托蒽醌-钥孔血蓝蛋白（MZ-KLH）偶联物免疫BALB/c小鼠，制备了一种对抗癌药物米托蒽醌（MZ，商品名诺维本）具有特异性的小鼠单克隆抗体（NO-1）。当用于间接竞争酶联免疫吸附测定（ELISA）时，NO-1能准确、可重复地检测混合人血清中0.25 - 50 ng/ml的MZ，在此范围内获得的标准曲线几乎呈线性。该测定法具有良好的重复性，批内变异系数（CV）在1.41%至7.02%之间，批间CV为3.45%。ELISA检测到的MZ水平与添加到混合人血清中的实际量的回归分析给出了非常好的相关系数r = 0.995。NO-1与比生群或柔红霉素均无交叉反应。对体重0.5 mg/kg静脉注射MZ的家兔进行了一项简单的药代动力学研究。用该测定法测得的家兔血清中MZ水平，家兔1和家兔2在15分钟时分别为82至170 ng/ml，家兔1在48小时时降至1.25 ng/ml，家兔2在120小时时降至检测不到的水平。
