Gao Weimin
Department of Occupational and Environmental Health Sciences, School of Public Health, West Virginia University, Morgantown, WV, USA.
Methods Mol Biol. 2020;2102:163-176. doi: 10.1007/978-1-0716-0223-2_8.
Two-dimensional difference gel electrophoresis (2D-DIGE) remains to be one of the most popular and versatile methods of protein separation among many proteomics technologies. Similar to traditional two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the proteins are separated based on their charges and molecular weight by 2D-DIGE. Different from 2D-PAGE, proteins are pre-labeled with different fluorescent dyes, and different protein samples are run in one gel by this method. Therefore, 2D-DIGE not only carries the advantages of 2D-PAGE but also eliminates gel-to-gel variation and achieves high resolution, sensitivity, and reproducibility.
二维差异凝胶电泳（2D-DIGE）仍然是众多蛋白质组学技术中最流行、用途最广泛的蛋白质分离方法之一。与传统的二维聚丙烯酰胺凝胶电泳（2D-PAGE）类似，2D-DIGE通过蛋白质的电荷和分子量对其进行分离。与2D-PAGE不同的是，蛋白质预先用不同的荧光染料进行标记，通过这种方法不同的蛋白质样品可以在一块凝胶上进行电泳。因此，2D-DIGE不仅具有2D-PAGE的优点，还消除了凝胶间的差异，实现了高分辨率、高灵敏度和高重复性。
