二维差异凝胶电泳：一种基于凝胶的蛋白质组学蛋白质分析方法。

Two-Dimensional Difference Gel Electrophoresis: A Gel-Based Proteomic Approach for Protein Analysis.

作者信息

Gao Weimin

机构信息

Department of Occupational and Environmental Health Sciences, School of Public Health, West Virginia University, Morgantown, WV, USA.

出版信息

Methods Mol Biol. 2020;2102:163-176. doi: 10.1007/978-1-0716-0223-2_8.

DOI:10.1007/978-1-0716-0223-2_8

PMID:31989554

Abstract

Two-dimensional difference gel electrophoresis (2D-DIGE) remains to be one of the most popular and versatile methods of protein separation among many proteomics technologies. Similar to traditional two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the proteins are separated based on their charges and molecular weight by 2D-DIGE. Different from 2D-PAGE, proteins are pre-labeled with different fluorescent dyes, and different protein samples are run in one gel by this method. Therefore, 2D-DIGE not only carries the advantages of 2D-PAGE but also eliminates gel-to-gel variation and achieves high resolution, sensitivity, and reproducibility.

摘要

二维差异凝胶电泳（2D-DIGE）仍然是众多蛋白质组学技术中最流行、用途最广泛的蛋白质分离方法之一。与传统的二维聚丙烯酰胺凝胶电泳（2D-PAGE）类似，2D-DIGE通过蛋白质的电荷和分子量对其进行分离。与2D-PAGE不同的是，蛋白质预先用不同的荧光染料进行标记，通过这种方法不同的蛋白质样品可以在一块凝胶上进行电泳。因此，2D-DIGE不仅具有2D-PAGE的优点，还消除了凝胶间的差异，实现了高分辨率、高灵敏度和高重复性。