Department of Poultry Production, Faculty of Agriculture, Damietta University, Damietta, Egypt.
Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura, Egypt.
J Anim Physiol Anim Nutr (Berl). 2020 Mar;104(2):539-548. doi: 10.1111/jpn.13316. Epub 2020 Jan 27.
This study investigated the effect of daily oral administration with allicin levels (0, 5 and 10 mg/kg of female body weight), 30 days pre-insemination, on reproductive performance in vivo and in vitro, immunity, and oxidative stress of rabbit does under high ambient temperature. Niliparous NZW does (n = 105) were randomly divided into three groups (35 in each) treated with 0, 5 and 10 mg allicin dissolved in 2 ml distilled water, respectively, for 30 days pre-insemination. At the end of treatment (30 days), does were artificially inseminated with fresh diluted semen of 20 fertile NZW bucks. Reproductive performance and ovulatory response parameters were determined. Serum biochemicals, enzyme activity, immunoglobulins (IgG and IgM) and antioxidant status were determined on day 30 of treatment. Serum progesterone and prolactin were determined pre-insemination (30 days of treatment), on 15 days of pregnancy and 7 days post-partum. Results showed that both allicin levels increased live litter size, and bunny viability rat and litter size at birth and weaning. Allicin levels increased ovulation rate and improved embryo quality. Number of total follicles decreased only with 10 mg allicin. Progesterone increased pre-insemination, 15 days of pregnancy and 7 days post-partum progesterone by allicin levels. Prolactin pre-insemination and on day 7 post-partum increased with 10 mg allicin. Serum total proteins, albumin, globulin, IgG and IgM increased, while glucose, aspartate and alanine aminotransaminases, and thiobarbituric acid reaction decreased by both allicin levels. In conclusion, the mechanism by which allicin administration 30 days pre-insemination to improve the reproductive performance of rabbit does is based on that allicin can play an important role, as a natural exogenous antioxidant, increasing immune response and reducing lipid peroxidation.
本研究调查了每日口服大蒜素水平（雌性体重的 0、5 和 10mg/kg），在授精前 30 天，对高温环境下母兔体内和体外生殖性能、免疫和氧化应激的影响。将无妊娠史的 NZW 母兔（n=105）随机分为三组（每组 35 只），分别用 0、5 和 10mg 大蒜素溶于 2ml 蒸馏水，在授精前 30 天处理。治疗结束时（30 天），母兔用 20 只健康 NZW 公兔的新鲜稀释精液人工授精。测定繁殖性能和排卵反应参数。在治疗的第 30 天测定血清生化指标、酶活性、免疫球蛋白（IgG 和 IgM）和抗氧化状态。在授精前（治疗的第 30 天）、妊娠 15 天和产后 7 天测定血清孕酮和催乳素。结果表明，大蒜素水平均提高了活产仔数、兔仔活力率和产仔数及断奶仔数。大蒜素水平提高了排卵率，改善了胚胎质量。只有 10mg 大蒜素水平降低了总卵泡数。孕酮在授精前、妊娠 15 天和产后 7 天均因大蒜素水平而增加。催乳素在授精前和产后 7 天因 10mg 大蒜素而增加。血清总蛋白、白蛋白、球蛋白、IgG 和 IgM 增加，而葡萄糖、天门冬氨酸和丙氨酸氨基转移酶以及硫代巴比妥酸反应产物减少。综上所述，大蒜素在授精前 30 天给药以提高母兔繁殖性能的机制在于，大蒜素作为一种天然外源性抗氧化剂，可以发挥重要作用，增加免疫反应，减少脂质过氧化。
