EB 病毒相关胃癌中异常甲基化差异表达的基因和通路。
Aberrantly methylated-differentially expressed genes and pathways in Epstein-Barr virus-associated gastric cancer.
机构信息
Tumor Etiology & Screening Department of Cancer Institute & General Surgery, the First Hospital of China Medical University, Shenyang 110001, PR China.
Key Laboratory of Cancer Etiology & Prevention in Liaoning Education Department, the First Hospital of China Medical University, Shenyang 110001, PR China.
出版信息
Future Oncol. 2020 Feb;16(6):187-197. doi: 10.2217/fon-2019-0649. Epub 2020 Jan 28.
To identify the methylated-differentially expressed genes (MDEGs) that may serve as diagnostic markers and therapeutic targets in Epstein-Barr virus-associated gastric cancer (EBVaGC) and to explore the methylation-based pathways for elucidating biological mechanisms of EBVaGC. Gene expression and methylation profiles were downloaded from GEO database. MDEGs were identified by GEO2R. Pathway enrichment analyses were conducted based on DAVID database. Hub genes were identified by Cytoscape, which were further verified by The Cancer Genome Atlas database. A total of 367 hypermethylated, lowly expressed genes were enriched in specific patterns of cell differentiation. 31 hypomethylated, highly expressed genes demonstrated enrichment in regulation of immune system process. After validation using The Cancer Genome Atlas database, seven genes were confirmed to be significantly different hub genes in EBVaGC. EBVaGC-specific MDEGs and pathways can be served as potential biomarkers for precise diagnosis and treatment of EBVaGC and provide novel insights into the mechanisms involved.
为了鉴定可能作为 Epstein-Barr 病毒相关胃癌（EBVaGC）诊断标志物和治疗靶点的甲基化差异表达基因（MDEGs），并探讨基于甲基化的途径，以阐明 EBVaGC 的生物学机制。从 GEO 数据库下载基因表达和甲基化谱。通过 GEO2R 鉴定 MDEGs。基于 DAVID 数据库进行通路富集分析。通过 Cytoscape 识别枢纽基因，并通过 The Cancer Genome Atlas 数据库进一步验证。共有 367 个高甲基化、低表达的基因在特定的细胞分化模式中富集。31 个低甲基化、高表达的基因在免疫系统过程的调节中富集。使用 The Cancer Genome Atlas 数据库验证后，确认有七个基因是 EBVaGC 中显著差异的枢纽基因。EBVaGC 特异性 MDEGs 和途径可作为 EBVaGC 精确诊断和治疗的潜在生物标志物，并为相关机制提供新的见解。
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