Laboratory for Water and Waste Treatment, Environmental and Sanitary Engineering Department, Santa Catarina State University, Lages, Santa Catarina, 88520-000, Brazil.
Biological Engineering Integrated Laboratory, Chemical and Food Engineering Department, Federal University of Santa Catarina, Campus Universitário Trindade, Florianópolis, Santa Catarina, 88040-900, Brazil.
J Environ Manage. 2020 Feb 15;256:109964. doi: 10.1016/j.jenvman.2019.109964. Epub 2019 Dec 13.
The contamination of water resources by nitrate is a global problem. Indeed, traditional treatment technologies are not able to remove this ion from water. Alternatively, biological denitrification is a useful technique for natural water nitrate removal. This study aimed to evaluate the use of glycerol as a carbon source for drinking water nitrate removal via denitrification in a reactor using microorganisms from natural biomass. The experiment was carried out in a continuous fixed bed reactor using immobilised microorganisms from the vegetal Phyllostachys aurea. The tests were started in batch mode to provide cells growth and further immobilisation on the support. Then, the treatment experiments were accomplished in an up-flow continuous reactor. Ethanol was used as the primary carbon source, and it was gradually replaced by glycerol. The C:N (carbon to nitrogen) ratio and the hydraulic residence time (HRT) were evaluated. It was possible to remove 98.14% of nitrate using a C:N ratio and HRT of 3:1 and 1.51 days, respectively. The results have demonstrated that glycerol is a potential carbon source for denitrification in a continuous reactor using immobilised cells from natural biomass.
水资源中硝酸盐的污染是一个全球性的问题。事实上，传统的处理技术无法从水中去除这种离子。相比之下，生物反硝化是一种从天然水中去除硝酸盐的有用技术。本研究旨在评估使用甘油作为碳源，通过使用来自天然生物质的微生物在反应器中进行反硝化作用来去除饮用水中的硝酸盐。该实验在连续固定床反应器中进行，使用来自植物刚竹的固定化微生物。实验首先在分批模式下进行，以提供细胞生长并进一步固定在载体上。然后，在升流式连续反应器中进行处理实验。使用乙醇作为主要碳源，并逐渐用甘油替代。评估了 C:N（碳氮比）和水力停留时间（HRT）。当 C:N 比和 HRT 分别为 3:1 和 1.51 天时，可以去除 98.14%的硝酸盐。结果表明，甘油是使用来自天然生物质的固定化细胞在连续反应器中进行反硝化作用的潜在碳源。
