Meunier M, Brebion P, Chêne N, Servely J L, Martinet L
Département de Physiologie Animale, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
J Endocrinol. 1988 Nov;119(2):287-92. doi: 10.1677/joe.0.1190287.
Mink anterior pituitaries were incubated in Medium 199 for up to 9 or 13 days. Biological activity of prolactin and GH was determined. Daily concentrations of prolactin and GH in the incubation medium were also measured by radioimmunoassay and radioreceptor assay. When females were kept under short days for several weeks before the experiment, a significant decrease in prolactin secretion by the anterior pituitary was observed as compared with that in females maintained under long days. In contrast, secretion of GH was not modified by the photoperiodic history of the animals. Pineal gland denervation by ablation of the superior cervical ganglia a few months before the experiment, or addition of melatonin to the incubation medium of anterior pituitaries from intact or ganglionectomized females, did not modify the secretion of prolactin and GH. The pituitary gland does not therefore seem to be a direct target site for melatonin in transducing the duration of daylength on the hypothalamo-pituitary axis.
将水貂的垂体前叶在199培养基中培养长达9天或13天。测定催乳素和生长激素的生物活性。还通过放射免疫分析和放射受体分析测量了培养基中催乳素和生长激素的每日浓度。在实验前将雌性动物置于短日照条件下数周，与处于长日照条件下的雌性动物相比，观察到垂体前叶催乳素分泌显著减少。相反，动物的光周期史对生长激素的分泌没有影响。在实验前几个月通过切除颈上神经节使松果体去神经支配，或者在完整或去神经节雌性动物的垂体前叶培养基中添加褪黑素，均未改变催乳素和生长激素的分泌。因此，垂体似乎不是褪黑素在下丘脑-垂体轴上传递日照时长信息的直接靶位点。
