Lopez J, Talamantes F
Life Sci. 1983 May 2;32(18):2103-6. doi: 10.1016/0024-3205(83)90098-x.
Long-term organ cultures of mouse anterior pituitaries provides a system for generating an enriched starting material for the purification of prolactin and growth hormone. Culturing 1 anterior pituitary per ml in serum-free medium for 12 days resulted in a total yield of 40.9 micrograms and 6.6 micrograms of prolactin and growth hormone, respectively. This yield represented a 1886% and 18% net increase over the control prolactin and growth hormone content in the unincubated pituitaries. Secondly, culturing of 1-5 anterior pituitaries per ml did not result in a negative feedback autoregulation in the culture system.
小鼠垂体前叶的长期器官培养提供了一个系统，用于生成用于纯化催乳素和生长激素的富集起始材料。在无血清培养基中每毫升培养1个垂体前叶12天，催乳素和生长激素的总产量分别为40.9微克和6.6微克。该产量相对于未培养的垂体中对照催乳素和生长激素的含量分别净增加了1886%和18%。其次，每毫升培养1 - 5个垂体前叶不会在培养系统中导致负反馈自动调节。
