Dixon L K, Wilkinson P J
Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey, U.K.
J Gen Virol. 1988 Dec;69 ( Pt 12):2981-93. doi: 10.1099/0022-1317-69-12-2981.
The genomes of African swine fever virus isolates collected from soft ticks (Ornithodoros moubata) inhabiting warthog burrows in four areas of Zambia were compared by restriction enzyme site mapping. Isolates from different areas showed considerable diversity. The regions of genomes that differed between isolates were distributed throughout the virus genome, although some more conserved regions were identified, such as the right-hand third of the genome. The genomes of seven isolates from neighbouring warthog burrows within Livingstone Game Park in southern Zambia were more similar to each other than those from different areas. However, a number of differences were observed even between the genomes of isolates from the same warthog burrow. The variation between these latter isolates probably resulted from point mutations located at various positions along the genome, in addition to small additions or deletions at both terminal regions. Restriction enzyme site mapping indicated that one isolate may have originated by earlier recombination between two distinguishable viruses.
通过限制性酶切位点图谱分析，对从赞比亚四个地区疣猪洞穴中栖息的软蜱（钝缘蜱）采集的非洲猪瘟病毒分离株的基因组进行了比较。来自不同地区的分离株表现出相当大的多样性。尽管鉴定出了一些更保守的区域，如基因组的右三分之一，但分离株之间不同的基因组区域分布在整个病毒基因组中。赞比亚南部利文斯通野生动物公园内相邻疣猪洞穴的七个分离株的基因组彼此之间比来自不同地区的分离株更相似。然而，即使在来自同一疣猪洞穴的分离株基因组之间也观察到了一些差异。这些后一种分离株之间的变异可能是由于沿着基因组不同位置的点突变，以及两个末端区域的小插入或缺失。限制性酶切位点图谱分析表明，一个分离株可能起源于两种可区分病毒之间的早期重组。
