Blasco R, Agüero M, Almendral J M, Viñuela E
Centro de Biología Molecular, Facultad de Ciencias, Universidad Autónoma, Canto Blanco, Madrid, Spain.
Virology. 1989 Feb;168(2):330-8. doi: 10.1016/0042-6822(89)90273-0.
An analysis of the SalI restriction pattern of African swine fever virus DNA showed that the SalI recognition sites did not change after more than 100 virus passages in porcine macrophages. The virus strain BA71V, obtained from the virus isolate BA71 by adaptation to grow in VERO cells, differed from the nonadapted virus in two deletions with a length of 2.5 and 7 kb located close to the DNA ends. A restriction analysis of several virus clones obtained from a naturally infected pig revealed length heterogeneity in both variable regions. A comparison of SalI restriction maps from 23 African swine fever virus field isolates (8 African, 11 European, and 4 American) has shown that the virus genome consists of a central region with a constant length of about 125 kb and two variable regions located close to the DNA ends with a length of 38-47 kb for the left DNA end, and 13-16 kb for the right DNA end. The total length of ASF virus DNA varied between 178 (BA71) and 189 (MOZ64) kb. The 23 African swine fever virus isolates were classified into five groups, according to the arrangement of the SalI sites in the central region. Four groups contained only African isolates, whereas all the European and American isolates belonged to the same group. This distribution of isolates suggests that all non-African virus field isolates have a common origin.
对非洲猪瘟病毒DNA的SalI限制性酶切图谱分析表明，在猪巨噬细胞中传代100多次后，SalI识别位点并未改变。从病毒分离株BA71经适应在VERO细胞中生长而获得的病毒株BA71V，与未适应的病毒相比，在靠近DNA末端处有两个长度分别为2.5 kb和7 kb的缺失。对从一头自然感染猪中获得的几个病毒克隆进行的限制性分析显示，两个可变区均存在长度异质性。对23个非洲猪瘟病毒野外分离株（8个非洲株、11个欧洲株和4个美洲株）的SalI限制性酶切图谱比较表明，病毒基因组由一个长度约125 kb的恒定中央区和两个靠近DNA末端的可变区组成，左DNA末端可变区长38 - 47 kb，右DNA末端可变区长13 - 16 kb。非洲猪瘟病毒DNA的总长度在178（BA71）至189（MOZ64）kb之间变化。根据中央区SalI位点的排列，将23个非洲猪瘟病毒分离株分为五组。四组仅包含非洲分离株，而所有欧洲和美洲分离株属于同一组。这种分离株的分布表明，所有非非洲病毒野外分离株都有共同的起源。
