Higgins J A, Klei T R, Foil L D
Department of Entomology, Louisiana State University, Baton Rouge 70803.
J Am Mosq Control Assoc. 1988 Sep;4(3):242-7.
Culicoides variipennis were fed under controlled conditions on two ponies that exhibited seasonal changes in Onchocerca cervicalis microfilarial (mf) skin density and skin distribution. The seasonal changes did not radically affect mf ingestion. Flies were fed on the umbilicus of infected ponies by two methods: individual feeding in consecutive order at the same site, or by mass feeding. Linear regression analysis indicated that ingestion of microfilaria was independent of feeding time and engorged weight. In the individual feeding data, there was a trend toward an increased ingestion of mf as the feeding time increased. Mass feeding trials suggest early feedings by C. variipennis may influence mf ingestion rates of flies that feed on subsequent days. Of the 1,104 flies feeding on both ponies over a 2-year period, 220 (20%) ingested mf and 99 (9% of all engorged flies) ingested only one mf.
在可控条件下，用具有盘尾丝虫颈微丝蚴（mf）皮肤密度和皮肤分布季节性变化的两匹小马喂养库蠓。季节性变化并未从根本上影响mf摄取。通过两种方法在受感染小马的脐部喂养苍蝇：在同一部位按连续顺序逐个喂养，或批量喂养。线性回归分析表明，微丝蚴摄取与喂养时间和饱食重量无关。在逐个喂养数据中，随着喂养时间增加，mf摄取量有增加的趋势。批量喂养试验表明，库蠓早期进食可能会影响后续几天进食苍蝇的mf摄取率。在两年时间里，在两匹小马上进食的1104只苍蝇中，220只（20%）摄取了mf，99只（占所有饱食苍蝇的9%）仅摄取了一条mf。
