Hosek J E, Todd K S, Kuhlenschmidt M S
Department of Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana 61801.
J Protozool. 1988 Nov;35(4):531-2. doi: 10.1111/j.1550-7408.1988.tb04145.x.
Sporozoite extracts of E. vermiformis, E. stiedai, and E. tenella are rich in acid phosphatase activity. They contain specific enzyme activities equal to or greater than those reported for other highly virulent protozoan parasites. The absolute amount of enzyme activity per oocyst dramatically increases during sporulation of E. stiedai and E. vermiformis. Partial characterization of the acid phosphatase activity of E. vermiformis indicates that sporozoites account for greater than 92% of the total activity in sporulated oocysts, that the enzyme is resistant to inhibition by tartrate, and that it can be separated into two forms by anion exchange chromatography.
微小隐孢子虫、斯氏艾美耳球虫和柔嫩艾美耳球虫的子孢子提取物富含酸性磷酸酶活性。它们所含的特定酶活性等于或高于其他高毒力原生动物寄生虫所报道的酶活性。在斯氏艾美耳球虫和微小隐孢子虫的孢子形成过程中，每个卵囊的酶活性绝对量急剧增加。对微小隐孢子虫酸性磷酸酶活性的部分特性分析表明，子孢子占孢子化卵囊中总活性的92%以上，该酶对酒石酸盐抑制具有抗性，并且可以通过阴离子交换色谱法分离成两种形式。
