基于亲和肽的阻抗型生物传感器用于灵敏检测大肠杆菌 O157:H7。
Impedimetric biosensor fabricated with affinity peptides for sensitive detection of Escherichia coli O157:H7.
机构信息
State Key Laboratory of Bioreactor Engineering, Biomedical Nanotechnology Center, Shanghai Collaborative Innovation Center for Biomanufacturing, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
出版信息
Biotechnol Lett. 2020 May;42(5):825-832. doi: 10.1007/s10529-020-02817-0. Epub 2020 Jan 28.
OBJECTIVE
To effectively and conveniently detect pathogenic bacteria, this study aimed to develop label-free biosensors fabricated affinity peptides that can recognize targeted bacteria strains and enable precise quantitative detections.
RESULTS
A 12-mer peptide with high binding affinity toward Escherichia coli O157:H7 was discovered by biopanning of phage-displayed peptide library. The peptide modified with glycine residues (G) and one cysteine (C) residue at C-terminal, could self-assemble on gold electrodes, enabling electrochemical impedance spectroscopy (EIS) analysis for quantitative detection of E. coli O157:H7. This method showed a low detection limit of 20 CFU/mL and a liner range from 2 × 10 to 2 × 10 CFU/mL.
CONCLUSION
It appears that, by designing and optimizing the structures of peptides, such a strategy can be greatly promising in developing quick, sensitive and quantitative biosensor of pathogens.
目的
为了有效地、方便地检测病原菌，本研究旨在开发无标记的生物传感器，这些传感器由能够识别目标细菌株的亲和肽制成，从而实现精确的定量检测。
结果
通过噬菌体展示肽文库的生物淘选，发现了一种与大肠杆菌 O157:H7 具有高结合亲和力的 12 肽。该肽在 C 末端修饰了一个甘氨酸（G）和一个半胱氨酸（C）残基，可在金电极上自组装，从而可以进行电化学阻抗谱（EIS）分析，实现对大肠杆菌 O157:H7 的定量检测。该方法的检测下限低至 20 CFU/mL，线性范围为 2×10 到 2×10 CFU/mL。
结论
通过设计和优化肽的结构，这种策略似乎在开发快速、敏感和定量的病原体生物传感器方面具有很大的前景。
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