Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, 35100 Bornova, Izmir, Turkey.
Anticancer Agents Med Chem. 2020;20(9):1129-1139. doi: 10.2174/1871520620666200128114215.
6-Gingerol (6G) and 6-Shogaol (6S) are the main active components of ginger. 6-Gingerol is known for its anti-metastatic and anti-invasive pharmacological activities on cancer cells, besides, 6-Shogaol also inhibits breast cancer cell invasion.
In this study, radioiodination (131I) of 6G and 6S was aimed. Additionally, it is aimed to monitor their incorporation behavior on breast cancer cell lines.
6-Gingerol was isolated from the fresh ginger-roots extract, additionally, dehydrated to obtain 6-Shogaol. 6G and 6S were radioiodinated using iodogen method. Quality control studies of radioiodinated ginger compounds (6G and 6S) were performed by thin layer radio-chromatography. In vitro studies of radioiodinated ginger compounds on MCF-7 and MDA-MB-231 cells were performed with incorporation assays.
6-Gingerol and 6-Shogaol were radioiodinated (131I-6G and 131I-6S) in high yields over 95%. 131I-6S demonstrated higher incorporation values than 131I-6G on MDA-MB-231 cells. Incorporation behavior of 131I-6G and 131I-6S was similar to MCF-7 cells.
It has been observed that ginger compounds were radioiodinated successfully and 131I-6S have a noteworthy incorporation on MDA-MB-231 cells which is a known breast carcinoma cell line with highly invasive characteristics.
6-姜酚（6G）和6-姜烯酚（6S）是生姜的主要活性成分。6-姜酚具有抗转移和抗侵袭的抗癌细胞药理学活性，此外，6-姜烯酚也能抑制乳腺癌细胞侵袭。
本研究旨在对 6G 和 6S 进行放射性碘标记，并监测它们在乳腺癌细胞系中的掺入行为。
从新鲜生姜根提取物中分离出 6-姜酚，然后进行脱水得到 6-姜烯酚。采用碘代法对 6G 和 6S 进行放射性碘标记。用薄层层析放射色谱法对放射性碘标记的姜化合物（6G 和 6S）进行质量控制研究。用掺入试验对 MCF-7 和 MDA-MB-231 细胞进行放射性碘标记姜化合物的体外研究。
6-姜酚和 6-姜烯酚均以超过 95%的高产率进行放射性碘标记（131I-6G 和 131I-6S）。131I-6S 在 MDA-MB-231 细胞中的掺入值高于 131I-6G。131I-6G 和 131I-6S 的掺入行为与 MCF-7 细胞相似。
成功地对生姜化合物进行了放射性碘标记，并且 131I-6S 在具有高度侵袭特性的已知乳腺癌细胞系 MDA-MB-231 细胞中具有显著的掺入作用。
Zotero 插件