Zoology department, Faculty of Science, Assiut University, 71516 Assiut, Egypt.
Photochem Photobiol Sci. 2020 Feb 19;19(2):261-273. doi: 10.1039/c9pp00236g.
Ultraviolet radiation is an ecological factor that directly affects terrestrial organisms through suppression of immunity or damage to internal organs. The present study assessed the effects of ultraviolet A (UVA) radiation on the kidneys of both wild-type (WT) and p53-deficient medaka (Oryzias latipes) and evaluated which strain was more resistant to the effects of UVA. Fish were divided into four groups: control group 1 (Cwt and Cp53), kept for 3 days without UVA exposure; group 2 (1wt and 1p53), fish exposed daily to UVA for 1 h day-1 for 3 days; group 3 (2wt and 2p53), fish exposed daily to UVA for 2 h day-1 for 3 days; and group 4 (3wt and 3p53), fish exposed daily to UVA for 3 h day-1 for 3 days. Samples of tissues were obtained 24 h after UVA exposure. The most obvious histopathological changes induced by UVA radiation in kidney tissues of both strains of medaka (WT and p53-deficient) were high levels of vacuolation of tubular cells followed by necrosis. The tubular segments lost their normal shape which appeared like a network structure and their cells with clear cytoplasm. Necrosis of lymphoid tissues and spots of brown pigmentation (possibly melanomacrophages) were sporadically seen in interstitial lymphoid tissues, while shrinkage of glomeruli, diminution of periodic acid-Schiff staining, and increased amount of collagenous fibers were observed. Our results confirmed the harmful effects of UVA radiation on kidney tissues of both WT and p53-deficient medaka. However, WT medaka was affected more than p53-deficient medaka.
紫外线辐射是一种通过抑制免疫或损害内脏器官而直接影响陆地生物的生态因素。本研究评估了紫外线 A（UVA）辐射对野生型（WT）和 p53 缺陷型斑马鱼（Oryzias latipes）肾脏的影响，并评估了哪种品系对 UVA 的影响更具抵抗力。将鱼分为四组：对照组 1（Cwt 和 Cp53），无 UVA 暴露培养 3 天；组 2（1wt 和 1p53），每天暴露于 UVA 1 小时，连续 3 天；组 3（2wt 和 2p53），每天暴露于 UVA 2 小时，连续 3 天；组 4（3wt 和 3p53），每天暴露于 UVA 3 小时，连续 3 天。在 UVA 暴露后 24 小时获得组织样本。UVA 辐射对两种斑马鱼（WT 和 p53 缺陷型）肾脏组织引起的最明显的组织病理学变化是肾小管细胞高度空泡化，随后发生坏死。肾小管段失去正常形态，呈网状结构，细胞质清晰。在间质淋巴组织中偶尔可见淋巴细胞坏死和棕色色素斑点（可能是黑色素巨噬细胞），而肾小球萎缩、过碘酸-Schiff 染色减少和胶原纤维增多。我们的结果证实了 UVA 辐射对 WT 和 p53 缺陷型斑马鱼肾脏组织的有害影响。然而，WT 斑马鱼比 p53 缺陷型斑马鱼受影响更大。
