Department of Respiratory Medicine and Allergology, Hospital de la Santa Creu i Sant Pau, Institute of Sant Pau Biomedical Research (IBB Sant Pau), Department of Medicine of Universitat Autònoma de Barcelona (UAB), CIBER of Respiratory Diseases (CIBERES), Barcelona, Spain.
PLoS One. 2020 Jan 29;15(1):e0228045. doi: 10.1371/journal.pone.0228045. eCollection 2020.
Most patients with nonallergic asthma have normal serum immunoglobulin E (IgE) levels. Recent reports suggest that total and aeroallergen-specific IgE levels in induced sputum may be higher in nonallergic asthmatics than in healthy controls. Our objective is to compare total and dust-mite specific (Der p 1) IgE levels in induced sputum in allergic and nonallergic asthmatics and healthy controls.
Total and Der p 1-specific IgE were measured in induced sputum (ImmunoCAP immunoassay) from 56 age- and sex-matched asthmatics (21 allergic, 35 nonallergic) and 9 healthy controls. Allergic asthma was defined as asthma with a positive prick test and/or clinically-significant Der p 1-specific serum IgE levels.
Patients with allergic asthma presented significantly higher total and Der p 1-specific serum IgE levels. There were no significant between-group differences in total sputum IgE. However, Der p 1-specific sputum IgE levels were significantly higher (p = 0.000) in the allergic asthmatics, but without differences between the controls and nonallergic asthmatics. Serum and sputum IgE levels were significantly correlated, both for total IgE (rho = 0.498; p = 0.000) and Der p 1-specific IgE (rho, 0.621; p = 0001).
Total IgE levels measured in serum and induced sputum are significantly correlated. No significant differences were found between the differents groups in total sputum IgE. Nevertheless, the levels of Der p 1-specific sputum IgE levels were significantly higher in the allergic asthmatics, but without differences between the controls and nonallergic asthmatics. Probably due to the lack of sensitivity of the test used, but with the growing evidence for local allergic reactions better methods are need to explore its presence. The Clinical Trials Identifier for this project is NCT03640936.
大多数非过敏性哮喘患者的血清免疫球蛋白 E（IgE）水平正常。最近的报告表明，诱导痰中的总 IgE 和变应原特异性 IgE 水平在非过敏性哮喘患者中可能高于健康对照组。我们的目的是比较过敏性和非过敏性哮喘患者以及健康对照组诱导痰中的总 IgE 和尘螨特异性（Der p 1）IgE 水平。
对 56 例年龄和性别匹配的哮喘患者（21 例过敏性，35 例非过敏性）和 9 例健康对照者的诱导痰（免疫捕获免疫分析法）中总 IgE 和 Der p 1 特异性 IgE 进行了测量。过敏性哮喘的定义为皮试阳性和/或临床显著的 Der p 1 特异性血清 IgE 水平。
过敏性哮喘患者的总 IgE 和 Der p 1 特异性血清 IgE 水平显著升高。各组间总痰 IgE 无显著差异。然而，过敏性哮喘患者的 Der p 1 特异性痰 IgE 水平显著升高（p = 0.000），但对照组和非过敏性哮喘患者之间无差异。血清和痰 IgE 水平显著相关，总 IgE（rho = 0.498；p = 0.000）和 Der p 1 特异性 IgE（rho，0.621；p = 0001）均如此。
血清和诱导痰中测得的总 IgE 水平显著相关。在总痰 IgE 方面，各组间无显著差异。然而，过敏性哮喘患者的 Der p 1 特异性痰 IgE 水平显著升高，但对照组和非过敏性哮喘患者之间无差异。可能是由于所用检测方法的敏感性不足，但随着局部过敏反应证据的增加，需要探索更好的方法来检测其存在。本项目的临床试验标识符为 NCT03640936。
