Zhao Weiyu, Cheng Han, Zhu Yu
Tianjin Medical College, Tianjin, China.
Department of Clinical Laboratory, Tianjin Huanhu Hospital, Tianjin, China.
J Clin Lab Anal. 2020 May;34(5):e23202. doi: 10.1002/jcla.23202. Epub 2020 Jan 29.
The level of homocysteine (Hcy) is significantly elevated in the plasma of patients with diabetes. The increased plasma Hcy level is positively correlated with the severity of the disease and is one of the important causes of diabetic complications.
We designed and synthesized a compound could reflect the level of Hcy based on rhodamine B, and the structure was verified by 1H-NMR and EI-HRMS. Then, the linearity, repeatability, selectivity, and cellar toxicity, the effects of the fluid viscosity and pH of compound on Hcy were measured; meanwhile, the response of Hcy level in the plasma of diabetic patients was detected.
This is a novel compound that has never been reported. The compound showed a satisfactory linear range and repeatability at the viscosity and pH of physiological fluid. In addition, the compound was capable of evading the interference from other amino acids and metal ions, and it exhibited high selectivity toward Hcy.
The compound showed increased responsiveness to plasma Hcy in patients with diabetes in comparison with healthy individuals and effectively reflected plasma Hcy levels in healthy individuals and diabetic patients. Therefore, the compound is expected to be used in the diagnosis of diabetes mellitus.
糖尿病患者血浆中同型半胱氨酸（Hcy）水平显著升高。血浆Hcy水平升高与疾病严重程度呈正相关，是糖尿病并发症的重要原因之一。
我们基于罗丹明B设计并合成了一种能反映Hcy水平的化合物，其结构通过1H-NMR和EI-HRMS进行了验证。然后，测定了该化合物的线性、重复性、选择性、细胞毒性以及化合物的流体粘度和pH对Hcy的影响；同时，检测了糖尿病患者血浆中Hcy水平的反应。
这是一种从未被报道过的新型化合物。该化合物在生理流体的粘度和pH条件下表现出令人满意的线性范围和重复性。此外，该化合物能够规避其他氨基酸和金属离子的干扰，对Hcy表现出高选择性。
与健康个体相比，该化合物对糖尿病患者血浆Hcy的反应性增强，并有效反映了健康个体和糖尿病患者的血浆Hcy水平。因此，该化合物有望用于糖尿病的诊断。
