适体连接的自组装 FRET 闪烁物用于活癌细胞中的 microRNA 成像。

Aptamer-tethered self-assembled FRET-flares for microRNA imaging in living cancer cells.

机构信息

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, China.

出版信息

Chem Commun (Camb). 2020 Feb 25;56(16):2463-2466. doi: 10.1039/c9cc09919k.

DOI:10.1039/c9cc09919k

PMID:31996881

Abstract

We report an aptamer-tethered, self-assembled DNA nanowire as a multivalent vehicle for the intracellular delivery of FRET flares. The FRET flares are bound to the nanowire and fluorescently labeled donors and acceptors at two ends, respectively. In the absence of targets, the flares are captured by binding with the nanowires, separating the donor and acceptor (low FRET). However, in the presence of target miRNAs, the flares are displaced from the nanowire, subsequently forming hairpin structures that bring the donor and acceptor into close proximity (high FRET).

摘要

我们报道了一种适体连接的自组装 DNA 纳米线，作为一种多价载体，用于细胞内传递 FRET 闪烁物。FRET 闪烁物分别结合在纳米线的两端，并带有荧光标记的供体和受体。在没有靶标的情况下，闪烁物与纳米线结合被捕获，使供体和受体分离（低 FRET）。然而，在存在靶 miRNA 的情况下，闪烁物从纳米线中被置换出来，随后形成发夹结构，使供体和受体靠近（高 FRET）。