National Engineering Research Center of Navel Orange, Gannan Normal University, Ganzhou, China.
South China Botanical Garden, Chinese Academy of Science, Guangzhou, China.
J Sci Food Agric. 2020 Apr;100(6):2664-2674. doi: 10.1002/jsfa.10297. Epub 2020 Feb 21.
Newhall navel orange (Citrus sinensis Osbeck cv. Newhall) is the major navel orange cultivar planted in China. Almost all Newhall navel orange peels produced in that country are currently discarded, which is not only harmful to the environment but also a waste of resources. It is therefore necessary to carry out research to explore the utilization potential of this resource.
A 95% ethanol extract (EE) of Newhall orange peel was prepared and fractionated into three subextracts: petroleum ether extract (PEE), ethyl acetate extract (EtOAcE), and water extract (WE) by simple liquid / liquid extractions. These four extracts were then subjected to antioxidant, antibacterial, and tyrosinase inhibition assays. Interestingly, EtOAcE was significantly superior to all other three extracts, exhibiting the best biological effects. The total polyphenol content (TPC), total flavonoid content (TFC), and primary individual flavonoids of these four extracts were analyzed and compared. This was followed by principal component analysis (PCA) and the Pearson's correlation test. The result indicates that the primary bioactive compounds responsible for the biological effects of the EtOAcE are sinensetin, 4',5,6,7-tetramethoxyflavone, nobiletin, 3,3',4',5,6,7-hexamethoxyflavone, and narirutin. In view of its easy preparation and potent biological effects, EtOAcE might demonstrate excellent application potential in various industrial areas.
This study successfully identified EtOAcE as a potent naturally occurring antioxidant, antibacterial, and tyrosinase inhibitory agent, which might add value to the utilization of Newhall navel orange peel in the food, cosmetic, and pharmaceutical industries. © 2020 Society of Chemical Industry.
纽荷尔脐橙（Citrus sinensis Osbeck cv. Newhall）是中国主要种植的脐橙品种。该国生产的几乎所有纽荷尔脐橙皮目前都被丢弃，这不仅对环境有害，也是资源的浪费。因此，有必要开展研究，探索这种资源的利用潜力。
用 95%乙醇从纽荷尔橙皮中提取并通过简单的液-液萃取分为三个亚提取物：石油醚提取物（PEE）、乙酸乙酯提取物（EtOAcE）和水提取物（WE）。然后对这四种提取物进行抗氧化、抑菌和酪氨酸酶抑制试验。有趣的是，EtOAcE 明显优于其他三种提取物，表现出最好的生物效应。分析比较了这四种提取物的总多酚含量（TPC）、总黄酮含量（TFC）和主要单体黄酮。然后进行主成分分析（PCA）和 Pearson 相关检验。结果表明，EtOAcE 产生生物效应的主要生物活性化合物是橙皮苷、4',5,6,7-四甲氧基黄酮、川陈皮素、3,3',4',5,6,7-六甲氧基黄酮和诺米林。鉴于其易于制备和强大的生物效应，EtOAcE 可能在食品、化妆品和制药等各个工业领域具有出色的应用潜力。
本研究成功地鉴定出 EtOAcE 是一种有效的天然抗氧化剂、抑菌剂和酪氨酸酶抑制剂，这可能为纽荷尔脐橙皮在食品、化妆品和制药行业的利用增加价值。© 2020 英国化学学会。
