人孕酮受体的纯化、单克隆抗体制备及结构分析。
Purification, monoclonal antibody production and structural analyses of human progesterone receptors.
作者信息
Horwitz K B
机构信息
University of Colorado Health Sciences Center, Denver, CO 80220.
出版信息
J Steroid Biochem. 1988 Oct;31(4B):573-8. doi: 10.1016/0022-4731(88)90008-8.
PMID:3199807
Abstract
Progesterone receptors (PR) from human breast cancer cells have been purified, and used as antigens to generate anti-PR monoclonal antibodies. Immunologic and in situ photoaffinity labeling methods were then used to study receptor structure, and we conclude that native human PR consist of two independent 8S receptors: one 8S subtype contains B-proteins (120,000 kDa) and the other contains A-proteins (94,000 kDa).
摘要
已从人乳腺癌细胞中纯化出孕激素受体（PR），并将其用作抗原以产生抗PR单克隆抗体。然后使用免疫和原位光亲和标记方法研究受体结构，我们得出结论，天然人PR由两种独立的8S受体组成：一种8S亚型含有B蛋白（120,000 kDa），另一种含有A蛋白（94,000 kDa）。
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