Muller L M, Phillipou G
Department of Clinical Chemistry, Queen Elizabeth Hospital, Woodville, South Australia.
J Steroid Biochem. 1988 Dec;31(6):979-82. doi: 10.1016/0022-4731(88)90341-x.
The urinary levels of seven steroids, 5 alpha-androstane-3 alpha,17 beta-diol, 5 beta-androstane-3 alpha,17 beta-diol, androsterone, etiocholanolone, tetrahydrocortisone, tetrahydrocortisol and allotetrahydrocortisol were measured in both normal (n = 18) and hirsute (n = 24) women. The results confirmed 5 alpha-androstane-3 alpha,17 beta-diol as the most significant steroid with respect to discrimination between hirsute and normal subjects. Investigation of the inter-steroid relationships, using multivariate techniques established that the mode of steroid metabolism was different between the two groups. Whereas in normal women the strong correlation amongst all the androgen metabolites inferred a predominant hepatic route to 5 alpha-androstane-3 alpha,17 beta-diol formation, the same analogy was not applicable to the hirsute subjects. Excellent agreement was found for the predicted vs actual excretion of 5 alpha-androstane-3 alpha,17 beta-diol in normal women, based on a regression model involving the six other steroids as independent variables. When the same model was used for estimation of 5 alpha-androstane-3 alpha,17 beta-diol levels in thirteen hirsute subjects, misclassified as "normal", 50% gave values which were considerably less than actually measured. It is suggested that this discrepancy, with respect to these hirsute subjects is a reflection of extrahepatic production of 5 alpha-androstane-3 alpha,17 beta-diol due to increased 5 alpha-reductase activity.
对18名正常女性和24名多毛女性的尿液中七种甾体激素水平进行了测定，这七种甾体激素分别是5α-雄烷-3α,17β-二醇、5β-雄烷-3α,17β-二醇、雄酮、本胆烷醇酮、四氢皮质醇、四氢皮质素和别四氢皮质醇。结果证实，就区分多毛和正常受试者而言，5α-雄烷-3α,17β-二醇是最显著的甾体激素。运用多变量技术对甾体激素之间的关系进行研究，结果表明两组之间甾体激素的代谢模式不同。在正常女性中，所有雄激素代谢产物之间的强相关性表明，5α-雄烷-3α,17β-二醇的形成主要通过肝脏途径，而这一规律并不适用于多毛受试者。基于以其他六种甾体激素作为自变量的回归模型，正常女性5α-雄烷-3α,17β-二醇的预测排泄量与实际排泄量之间具有极佳的一致性。当使用同一模型对13名被误分类为“正常”的多毛受试者的5α-雄烷-3α,17β-二醇水平进行估计时，50%的受试者得出的值远低于实际测量值。这表明，对于这些多毛受试者而言，这种差异反映了由于5α-还原酶活性增加，5α-雄烷-3α,17β-二醇的肝外生成。
