Pongrac Paula, Fischer Sina, Thompson Jacqueline A, Wright Gladys, White Philip J
Ecological Science Group, The James Hutton Institute, Dundee, United Kingdom.
Low and Medium Energy Physics, Jožef Stefan Institute, Ljubljana, Slovenia.
Front Plant Sci. 2020 Jan 9;10:1645. doi: 10.3389/fpls.2019.01645. eCollection 2019.
Shoot zinc (Zn) concentration in is affected by soil Zn and phosphorus (P) supply. Most problematic is the negative impact of P fertilizers on Zn concentrations in crops, which makes balancing yield and mineral quality challenging. To evaluate early molecular mechanisms involved in the accumulation of large shoot Zn concentrations regardless of the P supply, two accessions differing in root architecture and root exudates were grown hydroponically for two weeks with different combinations of P and Zn supply. Ionome profiling and deep RNA sequencing of roots revealed interactions of P and Zn , without apparent phenotypic effects. In addition, increasing P supply did not reduce tissue Zn concentration. Substantial changes in gene expression in response to different P and/or Zn supplies in roots of both accessions ensured nutritionally sufficient P and Zn uptake. Numerous genes were differentially expressed after changing Zn or P supply and most of them were unique to only one accession, highlighting their different strategies in achieving nutrient sufficiency. Thus, different gene networks responded to the changing P and Zn supply in the two accessions. Additionally, enrichment analysis of gene ontology classes revealed that genes involved in lipid metabolism, response to starvation, and anion transport mechanisms were most responsive to differences in P and Zn supply in both accessions. The results agreed with previously studies demonstrating alterations in P and Zn transport and phospholipid metabolism in response to reduced P and Zn supply. It is anticipated that improved knowledge of genes responsive to P or Zn supply will help illuminate the roles in uptake and accumulation of P and Zn and might identify candidate genes for breeding high-yield-high-Zn brassicas.
地上部锌（Zn）浓度受土壤锌和磷（P）供应的影响。最成问题的是磷肥对作物锌浓度的负面影响，这使得平衡产量和矿物质质量具有挑战性。为了评估无论磷供应情况如何，参与积累高地上部锌浓度的早期分子机制，将两种根系结构和根系分泌物不同的材料进行水培，采用不同的磷和锌供应组合培养两周。根系的离子组分析和深度RNA测序揭示了磷和锌之间的相互作用，且无明显表型效应。此外，增加磷供应并未降低组织锌浓度。两种材料根系中因不同的磷和/或锌供应而导致的基因表达显著变化确保了对磷和锌的营养充足吸收。改变锌或磷供应后，大量基因差异表达，且大多数基因仅在一种材料中独特，突出了它们在实现养分充足方面的不同策略。因此，两种材料中不同的基因网络对变化的磷和锌供应做出了反应。此外，基因本体类别的富集分析表明，参与脂质代谢、饥饿响应和阴离子转运机制的基因对两种材料中磷和锌供应的差异反应最为敏感。这些结果与之前的研究一致，即表明磷和锌供应减少会导致磷和锌转运以及磷脂代谢发生改变。预计对响应磷或锌供应的基因的深入了解将有助于阐明其在磷和锌吸收与积累中的作用，并可能鉴定出用于培育高产高锌油菜的候选基因。
