Xie Shang-Qian, Xing Jian-Feng, Zhang Xiao-Ming, Liu Zhao-Yu, Luan Mei-Wei, Zhu Jie, Ling Peng, Xiao Chuan-Le, Song Xi-Qiang, Zheng Jun, Chen Ying
Key Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants, Hainan Key Laboratory for Biology of Tropical Ornamental Plant Germplasm, College of Forestry, Natural Rubber Cooperative Innovation Centre of Hainan Province & Ministry of Education of China, Hainan University, Haikou, China.
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
Front Genet. 2020 Jan 10;10:1288. doi: 10.3389/fgene.2019.01288. eCollection 2019.
-methyladenine (6mA) DNA modification has been detected in several eukaryotic organisms, where it plays important roles in gene regulation and epigenetic memory maintenance. However, the genome-wide distribution patterns and potential functions of 6mA DNA modification in woodland strawberry () remain largely unknown. Here, we examined the 6mA landscape in the genome by adopting single-molecule real-time sequencing technology and found that 6mA modification sites were broadly distributed across the woodland strawberry genome. The pattern of 6mA distribution in the long non-coding RNA was significantly different from that in protein-coding genes. The 6mA modification influenced the gene transcription and was positively associated with gene expression, which was validated by computational and experimental analyses. Our study provides new insights into the DNA methylation in .
N6-甲基腺嘌呤（6mA）DNA修饰已在几种真核生物中被检测到，它在基因调控和表观遗传记忆维持中发挥着重要作用。然而，6mA DNA修饰在森林草莓（Fragaria vesca）基因组中的全基因组分布模式和潜在功能仍 largely unknown。在这里，我们采用单分子实时测序技术检测了森林草莓基因组中的6mA图谱，发现6mA修饰位点广泛分布于森林草莓基因组中。长链非编码RNA中的6mA分布模式与蛋白质编码基因中的显著不同。6mA修饰影响基因转录，并与基因表达呈正相关，这一点通过计算分析和实验分析得到了验证。我们的研究为森林草莓中的DNA甲基化提供了新的见解。
