School of Life and Environmental Sciences, Deakin University, Waurn Ponds, Victoria 3216, Australia.
School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia.
Chem Commun (Camb). 2020 Feb 20;56(15):2240-2243. doi: 10.1039/c9cc09748a.
A fluorescent, naphthalimide-based, NADH mimic has been synthesised as a reversible, biocompatible, "on-off" probe for the detection of changes in intracellular redox environment (both oxidation and reduction). Interconversion was confirmed by means of electrochemistry and also 1H NMR, UV-vis and fluorescence spectroscopy. The reversibility was also successfully detected in A549 cells under simulated redox stress.
一种基于萘二甲酰亚胺的荧光 NADH 模拟物已被合成，作为一种可逆的、生物相容性的“开-关”探针，用于检测细胞内氧化还原环境的变化（氧化和还原）。通过电化学方法以及 1H NMR、UV-vis 和荧光光谱法证实了这种互变。在模拟氧化还原应激下，该化合物在 A549 细胞中的可逆性也得到了成功检测。
