Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Clinic for Ruminants, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Int J Syst Evol Microbiol. 2020 Mar;70(3):2115-2123. doi: 10.1099/ijsem.0.004027.
'' was originally described in 1912 by Noguchi but the name was not validly published and no type strain was designated. The taxon was not included in the Approved Lists of Bacterial Names and hence has no standing in nomenclature. Six strains positive in a '' phylogroup-specific PCR test were isolated from digital dermatitis (DD) lesions of cattle and further characterized and compared with the human strain '' ATCC 27087. Results of phenotypic and genotypic analyses including API ZYM, VITEK2, MALDI-TOF and electron microscopy, as well as whole genome sequence data, respectively, showed that they form a cluster of species identity. Moreover, this species identity was shared with ''-like strains reported in the literature to be regularly isolated from bovine DD. High average nucleotide identity values between the genomes of bovine and human '' were observed. Slight genomic as well as phenotypic variations allowed us to differentiate bovine from human isolates, indicating host adaptation. Based on the fact that this species is regularly isolated from bovine DD and that the name is well dispersed in the literature, we propose the species sp. nov., nom. rev. The species can phenotypically and genetically be identified and is clearly separated from other species. The valid species designation will allow to further explore its role in bovine DD. The type strain for sp. nov., nom. rev. is B43.1 (=DSM 110455=NCTC 14362) isolated from a bovine DD lesion in Switzerland.
''于 1912 年由野口首次描述，但该名称未有效发表，也未指定模式菌株。该分类群未包含在已批准的细菌名称列表中，因此在命名法中没有地位。从牛的数字性皮炎 (DD) 病变中分离出 6 株在 ''种特异性 PCR 检测中呈阳性的菌株，并对其进行了进一步的特征描述和与人源菌株 '' ATCC 27087 进行了比较。表型和基因型分析（包括 API ZYM、VITEK2、MALDI-TOF 和电子显微镜检查）以及全基因组序列数据的结果分别表明，它们形成了一个种的同一性聚类。此外，这种种的同一性与文献中报道的经常从牛 DD 中分离出的 ''样菌株共享。在牛和人 ''的基因组之间观察到高平均核苷酸同一性值。牛和人 ''之间的基因组和表型差异很小，允许我们区分牛源和人源分离株，表明宿主适应。基于该物种经常从牛 DD 中分离出来，并且该名称在文献中广泛传播的事实，我们建议将该物种重新命名为 sp. nov.，nom. rev.。该物种可以通过表型和遗传特征进行鉴定，并且与其他 ''种明显分离。有效的物种命名将有助于进一步探索其在牛 DD 中的作用。 sp. nov.，nom. rev.的模式菌株为 B43.1（=DSM 110455=NCTC 14362），从瑞士的牛 DD 病变中分离得到。
