Corder R, Pralong F, Turnill D, Saudan P, Muller A F, Gaillard R C
Dept. of Medicine, University Hospital, Geneva, Switzerland.
Life Sci. 1988;43(23):1879-86. doi: 10.1016/s0024-3205(88)80005-5.
Immunoreactive glucocorticoid receptors (GR) have previously been demonstrated in neuropeptide Y (NPY) neurones of the rat hypothalamus. To determine whether NPY synthesis is influenced by glucocorticoids, the effect of dexamethasone (DEX) on the levels of immunoreactive NPY in rat hypothalamic neurones was investigated in vivo and in vitro. Daily injections of DEX (0.1 mg/day) for 5 days increased the NPY content of the mediobasal hypothalamus in female rats by 117% (p less than 0.002). Primary cultures of hypothalamic neurones were also sensitive to the effect of glucocorticoids. Intracellular NPY levels were significantly increased (p less than 0.001) compared to control values by 151%, 222% and 268% when cultures were maintained in a defined serum free medium containing DEX 10(-9), 10(-8) and 10(-7) M respectively.
免疫反应性糖皮质激素受体（GR）先前已在大鼠下丘脑的神经肽Y（NPY）神经元中得到证实。为了确定NPY的合成是否受糖皮质激素影响，研究了地塞米松（DEX）在体内和体外对大鼠下丘脑神经元中免疫反应性NPY水平的作用。对雌性大鼠连续5天每日注射DEX（0.1毫克/天），可使下丘脑中间基底部的NPY含量增加117%（p<0.002）。下丘脑神经元的原代培养物也对糖皮质激素的作用敏感。当培养物分别在含有10^(-9)、10^(-8)和10^(-7) M DEX的限定无血清培养基中培养时，细胞内NPY水平与对照值相比显著增加（p<0.001），分别增加了151%、222%和268%。
