Instituto de Investigación Biomédica A Coruña (INIBIC)-Complejo Hospitalario Universitario A Coruña (CHUAC), Coruña, Spain.
Centro de Investigación Biomédica en Red (CIBER) de Enfermedades Cardiovasculares (CIBERCV), Madrid, Spain.
Methods Mol Biol. 2020;2110:73-81. doi: 10.1007/978-1-0716-0255-3_5.
In pig-to-primate xenotransplantation, flow cytometry assays allow the examination of antibody reactivity to intact antigens in their natural conformation and location on cell membranes. Here we describe in detail the procedures of two flow cytometry assays to measure the antibody-mediated complement-dependent cytotoxicity (CDC) response or serum levels of IgG and IgM xenoantibodies. This information is key for understanding the rejection process of vascularized xenografts and finding strategies to overcome it.
在猪到灵长类动物的异种移植中，流式细胞术检测可用于检查完整抗原在细胞膜上的天然构象和位置的抗体反应性。在这里，我们详细描述了两种流式细胞术检测方法的程序，用于测量抗体介导的补体依赖性细胞毒性（CDC）反应或 IgG 和 IgM 异种抗体的血清水平。这些信息对于理解血管化异种移植物的排斥过程和寻找克服该过程的策略至关重要。
