Khokhlova V A, Rosnitskiy P B, Tsysar S A, Buravkov S V, Sapozhnikov O A, Karzova M M, Khokhlova T D, Maxwell A D, Gaifullin N M, Kadrev A V, Okhobotov D A, Kamalov A A, Schade G R
Physics Faculty, Lomonosov Moscow State University, Moscow, Russia.
Center for Industrial and Medical Ultrasound, University of Washington, Seattle, USA.
Urologiia. 2019 Dec 31(6):67-73.
of the study: demonstrate the feasibility of non-invasive mechanical disintegration of human prostate tissue using pulsed high-intensity focused ultrasound (pHIFU), a method termed boiling histotripsy.
An ultrasound experimental system was developed for producing localized mechanical lesions in ex vivo biological tissue samples under ultrasound guidance. A series of experiments was carried out to create small single-focus lesions (volume < 2 mm3) and one large lesion (volume > 50 mm3) in ex vivo prostate tissue samples. After irradiation, two samples were bisected to visualize the region of destruction; the other tissue samples were examined histologically.
During pHIFU irradiation under B-mode ultrasound guidance, a region of increased echogenicity caused by formation of vapor-gas bubbles was visualized in the target region. After exposure, small and large lesions filled with a suspension of liquefied tissue were observed. Histological examination confirmed that the prostate tissue in the focal region was disintegrated into subcellular fragments.
A pilot study showed the feasibility of using boiling histotripsy as a non-invasive method for treating prostate diseases.
证明使用脉冲高强度聚焦超声（pHIFU，一种称为沸腾组织粉碎术的方法）对人体前列腺组织进行非侵入性机械粉碎的可行性。
开发了一种超声实验系统，用于在超声引导下在离体生物组织样本中产生局部机械性损伤。进行了一系列实验，在离体前列腺组织样本中制造小的单焦点损伤（体积<2立方毫米）和一个大的损伤（体积>50立方毫米）。照射后，将两个样本切成两半以观察破坏区域；对其他组织样本进行组织学检查。
在B模式超声引导下进行pHIFU照射期间，在目标区域可见由蒸汽气泡形成引起的回声增强区域。暴露后，观察到充满液化组织悬浮液的小损伤和大损伤。组织学检查证实，焦点区域的前列腺组织已分解为亚细胞碎片。
一项初步研究表明，沸腾组织粉碎术作为一种治疗前列腺疾病的非侵入性方法是可行的。
