Laboratory of Animal Ecophysiology, Biology Department, Faculty of Sciences, University of Sfax, Sfax, Tunisia.
EA 7462 Géoarchitecture_Territoires, Urbanisation, Biodiversité, Environnement, Biology Department, Faculty of Sciences, University of Western Brittany, CS, Brest Cdex, France.
Hum Exp Toxicol. 2020 Jun;39(6):855-869. doi: 10.1177/0960327120903490. Epub 2020 Jan 31.
The current study was aimed at exploring the protective efficacy of spirulina against the hemato-biochemical alterations and nephrotoxicity induced by lead (Pb). Female rats aged 12 weeks were treated for 4 weeks with Pb (0.344 g kg bw) associated or not with spirulina (5.3 g kg bw). Renal damage induced by Pb was related to a severe anemia, increases of oxidative stress-related parameters (thiobarbituric acid reactive substances (TBARS) (+29%), protein carbonyl (PCO) (+66.3%), and advanced oxidation protein product (AOPP) (+110%)), plasma lactate dehydrogenase (LDH) (+80%), creatinine and urea levels in plasma, and uric acid concentration in urine, as well as genotoxic changes (+89.3% and +60% for DNA and mRNA levels, respectively). Conversely, LDH and antioxidant enzyme activities in kidney were decreased, as well as the levels of plasma uric acid, and urinary creatinine and urea levels. Spirulina-supplemented rats exhibited normal peripheral blood and renal parameters and renal histology. It can be suggested that alleviates damages induced by Pb, thanks to its high phenolic content and antioxidant capacity.
本研究旨在探讨螺旋藻对铅（Pb）引起的血液生化改变和肾毒性的保护作用。12 周龄雌性大鼠用 Pb（0.344 g kg bw）处理 4 周，同时或不与螺旋藻（5.3 g kg bw）一起处理。Pb 引起的肾损伤与严重贫血、氧化应激相关参数增加（硫代巴比妥酸反应物质（TBARS）增加 29%，蛋白羰基（PCO）增加 66.3%，高级氧化蛋白产物（AOPP）增加 110%）、血浆乳酸脱氢酶（LDH）增加 80%，血浆肌酐和尿素水平以及尿液尿酸浓度升高，以及遗传毒性改变（DNA 和 mRNA 水平分别增加 89.3%和 60%）有关。相反，螺旋藻补充大鼠的外周血和肾功能参数以及肾脏组织学均正常。可以认为，由于其高酚含量和抗氧化能力，螺旋藻缓解了 Pb 引起的损伤。
