Shahat Abdelaaty A, Hassanein Heba D, Ullah Riaz, Alqahtani Ali S, Husseiny Husseiny A, Kowalczyk Alicja, Farrag Abdel-Razik H
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
Chemistry of Medicinal Plants Department, National Research Centre, 33 El Bohouth St. (Former El Tahrirst.), Dokki, P.O. 12622, Giza, Egypt.
Evid Based Complement Alternat Med. 2022 Jun 29;2022:8545695. doi: 10.1155/2022/8545695. eCollection 2022.
The goal of the current study was to assess the nephroprotective and cardioprotective potential of methanol extract (MOR-1), as well as its butanol (MOR-2) and aqueous (MOR-3) fractions against carbon tetrachloride (CCl)-induced nephro and cardio-toxicity. Cardiac function was assessed using the biochemical parameters lactate dehydrogenase (LDH) and creatinine kinase (CK). Renal function was examined using the biochemical parameters creatinine and uric acid. The levels of nonprotein sulfhydryls (NPSH) and malondialdehyde (MDA) were used as markers of oxidative strain. A dose of 100 and 200 mg/kg of butanol fraction given prior to CCl treatment significantly ( < 0.05 - 0.001) protected against elevated LDH and CK levels. Similarly, treatment with silymarin (10 mg/kg) and butanol fraction (100 and 200 mg/kg) significantly ( < 0.05 - 0.001) boosted total protein levels compared to CCl treatment alone. The silymarin (10 mg/kg) and butanol fraction (100 and 200 mg/kg) also provided a significant ( < 0.05 - 0.001) protective effect for MDA levels. Methanol extract (MOR-1) and butanol (MOR-2) showed significant results and were recommended for further pharmacological and screening for active constituents.
本研究的目的是评估甲醇提取物（MOR-1）及其正丁醇（MOR-2）和水相（MOR-3）馏分对四氯化碳（CCl）诱导的肾毒性和心脏毒性的肾保护和心脏保护潜力。使用生化参数乳酸脱氢酶（LDH）和肌酸激酶（CK）评估心脏功能。使用生化参数肌酐和尿酸检查肾功能。非蛋白巯基（NPSH）和丙二醛（MDA）的水平用作氧化应激的标志物。在CCl处理前给予100和200mg/kg剂量的正丁醇馏分可显著（<0.05 - 0.001）防止LDH和CK水平升高。同样，与单独的CCl处理相比，水飞蓟宾（10mg/kg）和正丁醇馏分（100和200mg/kg）处理显著（<0.05 - 0.001）提高了总蛋白水平。水飞蓟宾（10mg/kg）和正丁醇馏分（100和200mg/kg）对MDA水平也有显著（<0.05 - 0.001）的保护作用。甲醇提取物（MOR-1）和正丁醇（MOR-2）显示出显著结果，建议进一步进行药理研究和活性成分筛选。
