木质素过氧化物酶、类过氧化物酶、漆酶、靛基质酶和磺基水杨酸对苯二甲酸酯去除贝扎贝特、吉非贝齐、吲哚美辛、磺胺甲恶唑和双氯芬酸的评价。

Evaluation of bezafibrate, gemfibrozil, indomethacin, sulfamethoxazole, and diclofenac removal by ligninolytic enzymes.

机构信息

Departamento de Ingeniería en Sistemas Ambientales, Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional, Mexico City, Mexico.

Departamento de Biotecnología, Universidad Autónoma Metropolitana Iztapalapa, Mexico City, Mexico.

出版信息

Prep Biochem Biotechnol. 2020;50(6):592-597. doi: 10.1080/10826068.2020.1721532. Epub 2020 Jan 31.

DOI:10.1080/10826068.2020.1721532

PMID:32003284

Abstract

The laccase (Lac), manganese peroxidases (MnP), and lignin peroxidase enzymes produced by basidiomycete have been studied due to their potential in bioremediation, therefore, in this study, degradation of diclofenac (DCF), sulfamethoxazole (SMX), indomethacin (IND), gemfibrozil (GFB), and bezafibrate (BZF) by enzymes produced by , and grown in culture was evaluated. The degradation of drugs can mainly be attributed to MnP because a correlation between the activity of this enzyme and the degree of removal was found. The specific activity of Lac did not show correlation with drug removal, while lignin peroxidase was not expressed. showed the highest specific activity of MnP (387.6 ± 67.4 U/mg) and efficiency removal 90.2% of DCF, 72.62% of SMX, 60.76% of IND, 43.39% of GFB, and 32.59% of BZF) followed by . with specific activity of MnP of 55.9 ± 8.5 U/mg and 89.47% of DCF, 47.61% of GFB and 73% of IND were removed, had the lowest specific activity of 18 ± 1.3 U/mg and was able to remove only 42% of SMX and 10.59% of IND. In order to prove that MnP remove drugs instead of Lac, the pure Lac was tested and only degraded DCF.

摘要

由于担子菌产生的漆酶 (Lac)、锰过氧化物酶 (MnP) 和木质素过氧化物酶在生物修复方面的潜力，因此，本研究评估了在培养条件下由、和产生的酶对双氯芬酸 (DCF)、磺胺甲恶唑 (SMX)、吲哚美辛 (IND)、吉非贝齐 (GFB) 和苯扎贝特 (BZF) 的降解作用。药物的降解主要归因于 MnP，因为发现该酶的活性与去除程度之间存在相关性。Lac 的比活性与药物去除没有相关性，而木质素过氧化物酶没有表达。表现出最高的 MnP 比活性 (387.6 ± 67.4 U/mg)，并能有效去除 90.2%的 DCF、72.62%的 SMX、60.76%的 IND、43.39%的 GFB 和 32.59%的 BZF)，其次是。其 MnP 比活性为 55.9 ± 8.5 U/mg，能去除 89.47%的 DCF、47.61%的 GFB 和 73%的 IND。具有最低的比活性 18 ± 1.3 U/mg，仅能去除 42%的 SMX 和 10.59%的 IND。为了证明 MnP 去除药物而不是 Lac，测试了纯 Lac，发现它仅能降解 DCF。